Waltham, MA: Academic Press, 2002. [4] Dowd, K. Measuring Market Risk. West Sussex: John Wiley & Sons, 2005. [5] McNeil, A. and R. Frey. "Estimation of Tail Related Risk Measure for Heteroscedastic Financial Time Series: An Extreme Value Approach." Journal of Empirical Finance...
Briefly, quantity of extracted DNA was measured again by NanoDropTM ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). 10 ul of ligation mixture (5 μl of 2X Rapid Ligation buffer, 1 μl of pGEM-T Easy Vector, 1 μl of T4 DNA ligase and 3 μl of gel extracted...
2. To avoid the advances of Pleistocene ice sheets, many terrestrial animals extirpated or migrated, with some in the northern hemisphere dispersing to the south and occupying sites that served as refugia3. Even
DNA concentrations were measured with QubitTM dsDNA Broad Range Assay Kit (InvitrogenTM, Waltham, MA, USA) and 240 ng of each PCR product was pooled into a single tube for each plate (amplicon pool). Each pool contained 96 samples plus two negatives. The quantity of negative sample added ...
For the isolation of TGEV and PDCoV, the trypsin (Gibco, Waltham, MA, USA) concentration in DMEM was adjusted to 1 μg/mL and 10 μg/mL, respectively. We confirmed the successful isolation of the virus by cytopathogenic effect (CPE) and reverse transcription-polymerase chain reaction (RT-...
Sequence chromatograms were produced in an automatic sequencer (ABI 3130XL Genetic Analyzer, Thermo Fischer, Waltham, MA, USA) using Big Dye Terminator 3.1 (Applied Biosystems, Thermo Fischer, Waltham, MA, USA). All newly generated sequences were submitted to GenBank (NCBI). Sequences had ...
The concentration of a set of trace elements was determined by ICP-MS using the NexION 300X (Perkin Elmer Inc., Waltham, MA, USA). The analytical uncertainty was evaluated by the analysis of soil reference material NIST SRM 2711a (Montana soil). RSD was 5% for Li, Be, Mn, Ni, Ag,...
Total DNA concentrations were estimated using a NanoDrop® ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Eluted DNA samples were kept at −20 °C until further processing. 2.4. LAMP on DNA Extracts from Needles Each DNA extract from pine needles was tested by LAMP...
Microwave system MARS-5 (Thermo Fisher Scientific, Waltham, MA, USA) was used for digestion of hair samples by the program previously optimized for Hg analysis in biological objects (Table 2) [42]. We tested acid and acid–peroxide digestion and demonstrated applicability of acid–peroxide digest...