Histone H3 (3H1) Rabbit Monoclonal Antibody detects endogenous levels of total histone H3 protein, including the Histone H3 variant CENP-A. This antibody does not cross-react with other core histones. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, D. melanogaster, Zebrafish, Bovine, Do...
Figure 1. Treatment of COS cells with trichostatin A (TSA) increases the acetylation of Histone H3 at Lys9 detected by PathScan® Acetyl-Histone H3 (Lys9) Sandwich ELISA Kit #7121. TSA treatment does not affect the level of histone H3 that is detected by Western analysis. COS cells (70...
Juan Ausió, in Handbook of Cell Signaling (Second Edition), 2010 Introduction Histones are the main protein component of chromatin and they can be divided into two major groups: core histones and linker histones. Core histones: H2A, H2B, H3, and H4 have a rather simple structural ...
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Primary antibodies used were anti-H3K36me2 (Cell Signaling Technology, cat. no. 2901, 1:500), anti-H3K36me2 (Diagenode, cat. no. C15200182, 1:500), anti-H3K36me3 (Cell Signaling Technology, cat. no. 4909, 1:500), anti-H3K36me3 (Active Motif, cat. no. 61101, 1:1000), ...
In this study, we provide molecular and structural basis by which Spindlin1 acts in complex with C11orf84 to preferentially recognize non-canonical bivalent mark of trimethylated lysine 4 and lysine 9 present on the same histone H3 tail (H3K4me3K9me3). We demonstrate that C11orf84 binding ...
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It is possible that both over-methylation and under-methylation of the two histone markers, H3K9me3 and H3K27me3, are detrimental to the proliferation of osteosarcoma cells. Future studies will focus on the mechanism of cell proliferation inhibition by rMETase and MTX, involving alteration of ...
increased dependence on TORC1 signaling to maintain viability, an effect that ultimately reduces the chronological longevity of H3K37 mutant cells under limiting nutrient conditions.ConclusionsTORC1 and histone H3 collaborate to retain HMGBs within the nucleus to maintain cell homeostasis and promote ...
(two copies each of histone H2A, H2B, H3, and H4). Based on this, by the association with linker histones (histone H1), the nucleosome arrays are further organized into solenoid conformation and looping domain structures that occur in both interphase and metaphase chromatin. During cell ...