A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser...
2a). The small fraction of regulated sites is within the normal range of quantitative error in SILAC experiments. Supporting this notion, the fraction of sites showing a decrease and increase in acetylation is very similar. Sites directly acetylated due to transcription would be expected to be ...
Calf Thymus Histone Preparation; Hela whole cell extract This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skin General notes In immunofluorescence: Human cells: The antibody stains a subset of cellular chromatin that is characteristically DAPI-rich (condensed, he...
In ChIP, an antibody specific to the modification is conjugated to a magnetic bead, enabling immunoprecipitation of the histones with that modification. The DNA bound to that histone can then be observed with high-throughput DNA sequencing. Mapping the resulting sequences to the genome can then ...
Boxplot center line is median, box limits are upper and lower quartiles, whiskers are the 1.5X interquartile range, and individual points are outliers. g Schematic depicting CUT&RUN working principle involving antibody-targeted digestion of chromatin by ProteinAG-MNase fusion protein. h Pie chart...
Moreover, iron deficiency seems to affect the humoral immune response (part of the immune system responsible for controlling the antibody response to a pathogen through the activation and differentiation of B cells in plasma) via diminished activity aligned to the iron-dependent JHDMs and a H3K9 ...
(ab9050, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 ...
(FPKM > 0) with H3K27ac modifications in normal morula were used for comparison. Wilcoxon rank sum test is used. Boxes and whiskers represent the 25th/75th percentiles and 1.5× interquartile range, respectively.cGenome browser view of H3K27ac signal and RNA-seq signal of ...
Anti-GFP antibody (Roche; #11 814 460 001) and secondary anti-mouse IgG-HRP antibody (Cell Signaling; #7076 G) were used. Fluorescence microscopy To assess localization of GFP-tagged centromere proteins, we used fluorescence microscopy on a Zeiss Axioplan 2 Microscope System equipped with a ...
normal goat serum / 0.3M glycine was used to quench autofluorescence and block non- specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA ...