In this study, it was found that alkaline earth metal cations (Mg2+, Ca2+) and alkali metal cations (Li+, Na+, K+) and even nonmetal cations (e.g., NH4+, imidazole, guanidine, arginine, lysine) can precipitate multi-histidine-tagged proteins (at least two tags in a whole protein) ...
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each ...
Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems tha...
GDNF family proteins are distant members of the TGF-beta superfamily and contain a conserved seven cysteine motif found in the entire family. Human NTN encodes a 197 amino acid (aa) preproprotein with a 19 aa residue putative signal peptide and a 76 aa pro region. Proteolytic cleavage of ...
Ni Sepharose excel is precharged with nickel ions that are very strongly bound to a chelating ligand and is designed primarily for the purification of histidine-tagged proteins secreted from eukaryotic cell based expression syst...
The high magnetic saturation value (41.3 emu g1) and superparamagnetic property of these nanoparticle clusters lead to a more efficient magnetically recyclable separation of His-tagged proteins. We confirmed that the binding capacity and selective separation ability seen in the first separation were ...
Ni-Charged Resin FF has low Ni2+leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni Charged Resin FF the excellent choice for high performance purification of polyhistidine-tagged proteins....
The development of simple techniques for the separation and purification of recombinant proteins plays an important role in many of the advancements made in biotechnology and nanotechnology. Herein, we report an easy method for the efficient purification of polyhistidine affinity-tagged (His-tagged) pro...
5) Recombinant fusion proteins 重组融合蛋白类6) recombinant fusion protein 重组融合蛋白 1. Protective immunity in mice after immunized with recombinant fusion protein encoding p46 kDa antigen of Trichinella spiralis; 旋毛虫编码新生幼虫p46 kDa抗原基因重组融合蛋白对小鼠的免疫保护性研究 更多例句>> ...
It has long been known that histidine-tagged proteins easily bind to the surface of transition metal oxides. Recently, Professor Hyeon's Group (Seoul National University, Korea) reported the preparation of magnetic nanoparticles using iron oxide and nickel oxide and a technology of effectively separat...