Categories: Protein science | Troubleshooting It took weeks to clone your his-tagged fusion protein and several more weeks to optimize the expression of your construct. After performing a trial purification and running the collected fractions on a gel, you find that the his-tagged protein fl...
Purification Procedure—Hybrid Conditions...13 Troubleshooting...15 Appendix.
10.Troubleshooting15 11.Regeneratingthemedium18 12.Cleaning-in-Place(CIP)19 13.Storage20 14.Furtherinormation20 15.Orderinginormation20 p.3 1.Productdescription NiSepharose6FastFlowishighlystableandcompatiblewithawiderange ocommonadditives.Thishelpstomaintainbiologicalactivityandincrease ...
Preparation before purification 11 8. Purification procedure 13 9. Optimization 14 10. Troubleshooting 15 11. Regenerating the medium 18 12. Cleaning-in-Place (CIP) 19 13. Storage 20 14. Further information 20 15. Orde 6、ring information 20 p. 3 1. Product description Ni Sepharose 6 Fast...
9Troubleshooting Thefollowingtipsmaybeofassistance.Ifyouhaveanyfurther questionsaboutyourHisTrapHPcolumn,pleasevisit com/hitrap,contactourtechnicalsupport,or yourlocalrepresentative. Note:WhenusinghighconcentrationsofureaorGua-HCl, proteinunfoldinggenerallytakesce.Refoldingon- ...
Troubleshooting issues with his-tag binding His-tag purification learning center How to choose the right his-tagged purification resinDate: June 17, 2019 Author: Takara Bio Blog Team Categories: Protein science | Useful resources In the grand scheme of your research, the resin you use to p...
Ni-NTA resins have been the most common IMAC resin choice for 6xHis-tag protein purifications because of the four metal-binding sites on the chelate, which enables high-protein binding and low-metal ion leaching. Cobalt binds less strongly and therefore more discriminantly, enabling greater ...
Troubleshooting 15 11. Regenerating the medium 18 12. Cleaning-in-Place (CIP) 19 13. Storage 20 14. Further information 20 15. Ordering information 20 p. 2 1. Product description Ni Sepharose 6 Fast Flow is highly stable and compatible with a wide range of common additives. This helps to...
Expected ResultsTroubleshooting Related Product Information Protein Gel Electrophoresis NuPAGE® Share|Introduction InVision™ His-tag In-gel Stain is a ready-to-use, proprietary fluorescent stain that is specially ...
HisPur Cobalt resin utilizes proprietary tetradentate chelating resin charged with cobalt. This system recovers highly purified protein with lower imidazole concentrations and has low metal leeching properties. Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation...