Construction and Application of Prokaryotic Expression Plasmid Containing GST and His Tags GST-His双标签原核表达载体的构建及应用 2. Construction of an EIAV Infectious Molecular Clone with Six HIS-Tag; 带有HIS标签的重组EIAV感染性分子克隆的构建 3. Analysis and Realization of Transfusion Label Based...
Fast accurate construct design for all major molecular cloning techniques Validate sequenced constructs using powerful alignment tools Customize plasmid maps with flexible annotation and visualization controls Automatically generate a rich graphical history of every edit and procedure ...
RP: the plasmid (pET28a) that was inserted into BG gene. enzyme leads to the reduction of combination opportunity between enzyme and nanoparticles. Each enzyme molecule competes to combine with nanoparticles, which gives rise to the partial combination between enzyme and nanoparticles within the ...
19046 Qiagen Plasmid Buffer Set 1070.00 301105 Qiagen PolyFect Transfection Reagent (1 ml) 1310.00 301108 Qiagen PolyFect Transfection Reagent (100 ml) 97350.00 301107 Qiagen PolyFect Transfection Reagent (4 x 1 ml) 4730.00 34924 Qiagen Polypropylene Columns (1 ml) 1380.00 34964 Qiagen Polyp...
1.Objective:Adding His tag to pGEX-KG to construct prokaryotic expression plasmid containing GST and His tags,and to increase purity of purified fusion proteins.结果:构建了GST-His双标签原核表达载体,将ERβ编码片段克隆入该载体中,在原核生物中得到表达;分别用GST和His抗体进行Western blot分析,均可检测...
The resulting plasmid (pSM179) encodes the mature form of YedY with a 6 His-tag and the TEV motif ENLYFQ (for cleavage with TEV protease) at the N-terminus. The DNA fragment corresponding to the yedY signal sequence was PCR-amplified with the primers YedSS and RYedSS (Additional file...
State-of-the-art algorithm used for plasmid design (Gene synthesis). This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein. The big advantage of ordering our made-to-order protei...
The MBP moiety serves to enhance the solubility and promote the proper folding of its fusion partners, and the His tag facilitates their purification to homogeneity via an entirely generic protocol. Experimental design This protocol describes how to (i) construct a dual His6-MBP (HisMBP)-tagged...
State-of-the-art algorithm used for plasmid design (Gene synthesis). This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein. The big advantage of ordering our made-to-order prot...
上海玉博生物科技有限公司 主要致力于“Tetra-His Antibody, BSA-free (100 ug) Qiagen 34670”的生产销售。多年的“Tetra-His Antibody, BSA-free (100 ug) Qiagen 34670”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用户信赖