Labeling and detection method: Time to complete visualization: Cost per blot (USD)*: GoldiBlot™ His-tag Western Blot Kit 1 hour 15 minutes $22.20 Chromogenic detection: peroxidase-labeled anti-His tag antibody and metal-enhanced DAB substrate 2 hours 50 minutes $34.49 Chemiluminescent detection...
The combination of MicroScale Thermophoresis (MST) and near-native site-specific His-tag labeling enables simple, robust, and reliable determination of the binding affinity between proteins and ligands. To demonstrate its applicability for periplasmic proteins, we provide a detailed protocol for determinat...
There are different methods to check binding of ligands to the beads, including optical density (OD) measurement, fluorescent labeling, and radioactive labeling. For OD measurement, you would measure the OD of the ligand before immobilization to the beads and compare it with the ligand concentrat...
However, there are not any universal methods that can be applied to all proteins, and it is thus important to explore the chemical strategy for protein modification. In this paper, we developed a new reactive peptide tag/probe pair system for site-specific covalent protein labeling. This method...
Western blot of 6*His-tagged fusion protein with anti-6*HIS tag (66005-1-Ig) at various dilutions.View All Images (4) Recommended products: NewMulti-rAb Recombinant Secondary Antibodies FlexAble Antibody Labeling Kits -/ - Cat No. 66005-1-Ig Freight/Packing: - Country/Region Size/...
Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni(2+):nitrilotriacetic Acid (n)-fluorochrome conjugates J. Am. Chem. Soc., 123 (48) (2001), pp. 12123-12125 View in ScopusGoogle Scholar [16] S. Lata, A. Reichel, R. ...
Protein labeling Antibody production Animal studies The reactive core of a nickel-based resin has less strict spatial requirements, so it binds histidines located in places other than the protein's polyhistidine tag, resulting in higher yields and lower purity. Nickel-based resins are commonly availa...
Michael G.Acker, ...Jon R.Lorsch, inMethods in Enzymology, 2007 8Overexpression and Purification of Yeast eIF1A inE. coli Proteins expressed from pTYB2 and purified using the IMPACT system have an extraglycine residueon their C-termini. Likewise,fluorescent labelingof the target protein results ...
“The ‘snitch jacket’ technique–neutralizing a target by labeling him a ‘snitch’ or informant, so that he could no longer be trusted–was used in all COINTELPROS. The methods utilized ranged from having an authentic informant start a rumor about a target member, to anonymous letters or...
offunctionalproteomics(1,2).Variousmethodshave beenusedforstudyingprotein:proteininteractions. Theseincludeyeast,bacterialandmammaliantwo-/ three-hybridsystems,immunoaffinitypurifications, affinitytag-basedmethodsandmassspectrometry (reviewedin1–3). Thoughtheyeasttwo-hybridsystemisthemostpopular ...