His tag coding sequence 5145-5162 T7 Terminator 5230-5276 pET-N-His-C-His质粒(5301bp)的图谱如下: pET-N-His-C-His的多克隆位点的详细图谱如下: rbs NdeI His tag BamHI 5052 TAACTTTAAG AAGGAG ATAT ACATATG CAC CATCATCATC ATCAT GGATC ...
His tag coding sequence5145-5162 T7 Terminator5230-5276 pET-N-His-C-His质粒(5301bp)的图谱如下: pET-N-His-C-His质粒的详细图谱见说明书:https://www.beyotime.com/Manual/D2902 pET-N-His-C-His.pdf。 pET-N-His-C-His中没有的酶切位点(Restriction enzymes that do not cut pET-N-His-C-...
His tag coding sequence 5079-5096 Multiple cloning site(BamHI-XhoI) 5097-5144 His tag coding sequence 5145-5162 T7 Terminator 5230-5276pET-N-His-C-His质粒(5301bp)的图谱如下:pET-N-His-C-His质粒中推荐使用的测序引物序列如下:T7 primer(4988-5004):5′-TAATACGACTCACTATA-3′pET-R primer(52...
His tag coding sequence 5088-5105 PreScission recognition site sequence 5115-5138 SUMO tag coding sequence 5151-5441 Multiple cloning site(BamHI-XhoI) 5442-5486 T7 Terminator 5554-5600 pET-N-His-PreScission-SUMO质粒(5625bp)的图谱如下: pET-N-His-PreScission-SUMO中的单酶切位点(Restriction enzymes...
His tag coding sequence 5079-5096 TEV Protease recognition site sequence 5097-5117 QUEEN7µ 5124-6266 T7 Terminator 6364-6410 pET-N-His-TEV-QUEEN7µ质粒(6417bp)的图谱如下: pET-N-His-TEV-QUEEN7µ中没有的酶切位点(Restriction enzymes that do not cut pET-N-His-TEV-QUEEN7µ)包括: ...
His tag coding sequence 5088-5105 PreScission recognition site sequence 5115-5138 SUMO tag coding sequence 5151-5441 Multiple cloning site(BamHI-XhoI) 5442-5486 T7 Terminator 5554-5600 质粒图谱: 详细图谱: pET-N-His-PreScission-SUMO的多克隆位点的详细图谱如下: 酶切位点: A.pET-N-His-PreScission...
His tag coding sequence5145-5162 T7 Terminator5230-5276 pET-N-His-C-His质粒(5301bp)的图谱如下: pET-N-His-C-His的多*位点的详细图谱如下: rbsNdeIHis tagBamHI 5052TAACTTTAAGAAGGAGATATACATATGCACCATCATCATCATCATGGATC ATTGAAATTCTTCCTCTATATGTATACGTGGTAGTAGTAGTAGTACCTAG ...
His tag coding sequence 5088-5105 PreScission recognition site sequence 5115-5138 SUMO tag coding sequence 5151-5441 Multiple cloning site(BamHI-XhoI) 5442-5486 T7 Terminator 5554-5600pET-N-His-PreScission-SUMO的多克隆位点的详细图谱如下: pET-N-His-PreScission-SUMO中没有的酶切位点包括: AatII ...
pET-N-His-PreScission-SUMO 是一种用于表达N 端同时含有His 标签(His tag)和SUMO 标签(SUMO tag)双标签的目的蛋白的原核表达质粒。His 标签有利于通过亲和层析方法进行目的蛋白的纯化，而SUMO 标签则有利于改善目的蛋白的折叠，增加目的蛋白可溶性和产量。表达后的含有目的蛋白的融合蛋白可以通过PreScission Protease (...
His tag coding sequence PreScission recognition site sequence SUMO tag coding sequence WELQ site Multiple cloning site (PstI-XhoI) T7 Terminator pT7-N-His-SUMO-WELQ的多克隆位点的详细图谱如下: XbaI RBS NdeI 5026 AATTCCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT TTAAGGGGAG ATCTTT...