The cells in the control group were cultured in the medium containing 10% normal rat serum, and the cells in the other groups were cultured in the medium containing 10% jaundice rat serum. The cells in control group and JS group were not treated with drugs...
Here, it was proved that hiHeps were hypoxic at day 8 of culture, even though the seeding density were 4×10 cells/cm in the PET-loaded bioreactor. Hypoxia impaired hiHep functions, such as albumin secretion, urea synthesis and drug metabolism. HIF-1α up-regulated its downstream genes ...