Here, we developed and assessed a highly multiplexed amplicon sequencing assay for the detection of GM events based on a microfluidics platform and next-generation sequencing (NGS). To probe GM events comprehen
One sample is used to generate full-length 16S amplicon sequences using PCR and PacBio sequencing. The resulting sequence file is used to generate a list of probes, which are purchased from a commercial vendor. The other sample is used for imaging experiments. Fixed samples are hybridized ...
During assay validation, we performed two GS Junior sequencing runs to determine the accuracy of the HLA class I amplicons and DRB amplicon at different levels of multiplexing. When 116 amplicons were multiplexed, we unambiguously resolved 99%of class I alleles to four- or six-digit resolution, ...
JR Dupuis,FT Bremer,A Kauwe,M San Jose,L Leblanc,D Rubinoff,S Geib,https://doi.org/10.1101/213454 (2017) HiMAP: Robust phylogenomics from highly multiplexed amplicon sequencing. bioRxiv. Google Scholar 12. DL Hancock,A review of the fruit fly tribe Tephrellini ( Diptera : Tephritidae ...
Sequencing of 16S rRNA can be used for bacterial identification14, however is costly and time consuming, and therefore has limited clinical utility. Highly multiplexed commercial assays are available such as the FilmArray Blood culture identification panel (Biofire Diagnostics, US) and the Verigene Gra...
Advances in automated DNA sequencing technology have greatly increased the scale of genomic and metagenomic studies. An increasingly popular means of increasing project throughput is by multiplexing samples during the sequencing phase. This can be achiev
The processed Sensor-seq datasets generated in this study have been deposited in Zenodo [https://doi.org/10.5281/zenodo.13381000]. The raw RNA-seq and amplicon sequencing files generated in this study have been deposited in the Sequence Read Archive (SRA) under accession codeSRP540614. The cry...
genome. For example, maize has a very repetitive genome and wheat has A, B, D sub-genomes that share high similarity. In these cases, targeted amplicon sequencing using next-generation sequencing technologies may be an appropriate and cost-effective method to look for off-target mutations [52]...
Moreover, the Genexus System permits the sequencing of 32 multiplexed samples in less than 24 h, representing a useful method for SARS-CoV-2 surveillance during a pandemic event. The Genexus System could be useful to analyse in a few days, respect to the Sanger sequencing, several viral ...
c) amplifying the copies of the template nucleic acids comprising the MLTs using a multiplexed polymerase chain reaction (PCR), wherein the plurality of mutation-prone genomic regions are simultaneously amplified; d) sequencing all or a subset of the amplified template nucleic acids comprising the ...