A systematic approach for authentication of medicinal Patrinia species using an integration of morphological, chemical and molecular methods Article Open access 19 March 2024 Application of a modified tetra-primer ARMS–PCR assay for rapid Panax species identity authentication in ginseng products Articl...
resolution, single-dose PTCs, or even a single-time exposure, which are much less informative. In view of the above, there is growing interest in high-throughput methods that comprehensively analyze the effect of different physiological and pharmacological factors on the clock. We report here the ...
Method comparison with gradient-based C18 and HILIC methods Extracts of RBC (10 μL) and AS-3 supernatant (20 μL) were analyzed with the 3-min method and two gradient-based methods using a C18 or HILIC column.[19,20] The two alternative chromatographic methods consisted o...
In this work, we exploit the fact that the CALPHAD framework offers a simple pathway to address this limitation of current high-throughput methods. While the integration of basic first-principles data with experimental data in the construction of CALPHAD thermodynamic database is an established techni...
After a brief introduction and summary of various methods of asymmetric induction in organic photochemistry, the main part of the review covers the solid-s... JR Scheffer,W Xia - Springer Berlin Heidelberg 被引量: 94发表: 0年 Asymmetric intramolecular selenoetherification and selenolactonization us...
3. Methods 1. MACS富集PBMC中 B细胞 2. CD27+记忆B细胞富集 3. 抗原结合B细胞富集 4. 单细胞5‘ rna和VDJ测序 5. 单克隆抗体的体外表达 6. ELISA定量、单克隆抗体结合亲和力测定 7. ACE2竞争测定 8. 蛋白表达纯化、冷冻电镜 9. 细胞病变效应(CPE)实验 ...
sequencing actions, based on higher-throughput methods. The distribution of reads over contigs was quite even, but the coverage was low, with an average between 6 and 7 reads per contig and around 90% of the contigs with 10 reads or fewer (Table3, Additional file7: Figure S3). The ...
However, the conventional methods for evaluating these enzymes are time consuming and have often been regarded as a bottle neck in developing these enzymes as industrial biocatalysts. In the past few years, several high throughput screening methods have been developed for fast evaluation and ...
Functional genomics of plant resistance to these insects would greatly benefit from the availability of high-throughput, quantitative phenotyping methods. Results We have developed an automated video tracking platform that quantifies aphid feeding behaviour on leaf discs to assess the level of plant ...
However, due to the limited understanding of context-dependent structure–function relationships, the identification of functional riboswitches requires large-scale-screening of aptamer-effector-domain designs, which is hampered by the lack of suitable cellular high-throughput methods. Here we describe a...