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2i) we carried out ten bootstrap samplings, and extracted 500 frames for each sampling for each condition. For each of the 500-frame bootstraps, all the frames are aligned pairwise for the RMSD calculations, yielding a 500 × 500 matrix. The averages and standard deviations of the ...
Error bars are obtained from 1000 bootstrap iterations. From the median of this dwell time distribution, we determine an unwinding rate of 5.2 bp/s at 36 pN. A third class of DNA constructs is the DNA hairpin (Fig. 2c). The use of DNA hairpins has several important advantages compared...
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