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As the COVID-19 pandemic continues and new strains are being discovered every day, there is a rush in the world of science and medicine to uncover how to best prevent and treat those who have been affected. This is a worldwide problem, not solely isolated in one location. However, becaus...
also constructed. Further, the combination of TLS and NER was adopted by simultaneously overexpressingumuDCand inactivatinguvrBoruvrCto generate strains HS89 (ΔuvrB::PcpcB560-umuDC) and HS90 (ΔuvrC::PcpcB560-umuDC), respectively. Among the second-round recombinant strains, HS84 (ΔmutS::P...
Furthermore, to determine the potential enzyme synergy, the expression levels of AAS and MAO were optimized using three promoters of different strengths in a single-vector system. Among the nine engineered strains,E. coliAMA05-3 exhibited the optimal glutarate production in the shake flask fermenta...
An overall review of the structural behaviors of ultra-high-performance fiber-reinforced concrete (UHPFRC) elements subjected to various loading conditions needs to be conducted to prevent duplicate research and to promote its practical applications. Thu
NTA was performed on Zmel1 EVs diluted 10 times with sterile PBS, using a Nanosight NS300 (Malvern Instruments) or a ZetaView (Particle Metrix). The measurement was repeated three times. For density gradient analysis, EVs isolated in the 100,000 g pellet were loaded on top of a 5-40%...
Cells were then washed multiple times and resuspended in Staining Buffer (PBS, without Ca2+ and Mg2+, 0.5% BSA, 2 mM EDTA). Flow cytometry and cell sorting Isolated cells from tumors or LNs were incubated with primary antibodies for 30 min, on ice in the dark, and then washed and ...
and 11–71 times, respectively. The bacterial community structure after a high level of petroleum pollution changed because of proliferation of the cells that initially were able to decompose hydrocarbons, and in the second place, because proliferation of the cells that received these catabolic genes...
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Bacterial strains under the same species can exhibit different biological properties, making strain-level composition analysis an important step in understanding the dynamics of microbial communities. Metagenomic sequencing has become the major means for