With advances in sequencing and instrument technology, bioinformatics analysis is being applied to batches of massive cells at single-cell resolution. High-throughput single-cell sequencing can be utilized for multi-omics characterization of tumor cells, stromal cells or infiltrated immune cells to evalua...
High-throughput single cell sequencing using CEL-Seq2 on a nanoliter dispensing robotFor the detection of subtle transcriptome changes reflecting fate biases, an scRNA-seq method is required that maximizes both sensitivity and accuracy. Recent benchmarking has demonstrated that CEL-Seq2 optimized both ...
High-throughput single-cell sequencing can be utilized for multi-omics characterization of tumor cells, stromal cells or infiltrated immune cells to evaluate tumor progression, responses to environmental perturbations, heterogeneous composition of the tumor microenvironment, and complex intercellular interactions...
membrane-boundantigens.CelliGOisadropletmicrofluid- icssystemthatcombineshigh-throughputscreeningforIgG activity,usingfluorescence-basedin-dropletsingle-cellbio- assays 2 ,withsequencingofpairedantibodyVgenes,using in-dropletsingle-cellbarcodedreversetranscription.Weana- lyzedIgGrepertoirediversity,clonalexpansionand...
High-throughput single-cell DNA sequencing of acute myeloid leukemia tumors with droplet microfluidics. Genome Res. 2018, 28, 1345-1352. [CrossRef] [PubMed] P Maurizio,S Adam,T Sebastian,... - 《Genome Research》 被引量: 1发表: 2018年 High‐Throughput Single‐Cell, Single‐Mitochondrial DNA...
Single-cell sequencing: 10x Genomics platform, for high-throughput single-cell sequencing for transcriptomics and epigenetics analysis. NanoString: nCounter and GeoMx platforms, for high-throughput gene expression analysis and spatial biology. Standard Genomics Services ...
2019年8月27日,北京大学分子医学研究所,北京大学-清华大学生命科学联合中心研究员何爱彬研究组在Molecular Cell杂志在线发表题为“CoBATCH for High-Throughput Single-Cell Epigenomic Profiling”的文章,报道了一种新的具有普适性、易操作、高通量和高质量的单细胞ChIP-seq技术,并将其命名为CoBATCH(combinatorial barcodin...
Recently developed high-throughput single-cell RNA sequencing (scRNA-seq) techniques, such as Drop-seq (Macosko et al., 2015), use a microfluidic device to encapsulate cells in emulsified droplets, allowing for the profiling of hundreds or even thousands of cells in a single experiment. Despite...
throughput method to identify the spatial origin of cells assayed by single-cell RNA-sequencing within a tissue of interest. Our approach is based on comparing complete, specificity-weighted mRNA profiles of a cell with positional gene expression profiles derived from a gene expression atlas. We ...
particularly in older adults. To elucidate the effects of age on the B cell response to influenza vaccination, we carried out paired single-cell RNA-seq and BCR sequencing on enriched B cell samples prior to and 7 days following vaccination with standard-dose, seasonal inactivated influenza vaccin...