The qRT-PCR validation results of DEPDC1, hsa_circ_0034415 and miR-1298-5p involved in the network were consistent with the sequencing results, which provided important research evidence for further study of these RNAs. Conclusions: The new discovered circRNA/lncRNA-miRNA-mRNA network in RA ...
Using Illumina Hiseq 2500, a new generation of high-throughput sequencing platform, all DNA of microalgae can be DE Novo sequenced, and mRNA can be reverse transcribed and highly sequenced to study metabolic pathways and expression regulation. At present, this method has been applied to the histo...
Cell atlas projects and high-throughput perturbation screens require single-cell sequencing at a scale that is challenging with current technology. To enable cost-effective single-cell sequencing for millions of individual cells, we developed ‘single-cell combinatorial fluidic indexing’ (scifi). The ...
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barcoding strategy requires multiple reactions in 96-well plates, which is not a popular or sensitive platform for high-throughput single-cell RNA-seq. Moreover, the majority of mapped reads in both PETRI-seq15and microSPLiT16were rRNA even with mRNA enrichment, leading to considerable sequencing...
High-throughput sequencing technologies are now routinely being applied to a wide range of important topics in biology and medicine, often allowing researchers to address important biological questions that were not possible before. In this review, we discuss these innovative new approaches-including ...
In the present study, we performed low salinity challenge with twoCrassostreaspecies (C. gigasandC. hongkongensis), and conducted high-throughput sequencing of four small RNA libraries constructed from the gill tissues. A total of 202 and 87 miRNAs were identified fromC. gigasandC. hongkongensis,...
(CGr) leaves using high-throughput sequencing method. The potential miRNA target genes were predicted and annotated using the NR, Swiss-Prot, GO, COG, KEGG, KOG and Pfam databases. The main biological processes regulated by miRNAs were revealed. The miRNAs which potentially play important roles ...
High-throughput sequencing was employed to investigate the expression of miRNAs and their target genes in superior and inferior seeds of Aikang 58. Small RNA sequencing revealed 620 conserved and 64 novel miRNAs in superior grains, and 623 conserved and
Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples Measures of RNA abundance are important for many areas of biology and often obtained from high-throughput RNA sequencing methods such as Illumina sequence ... GP Wagner,K Kin,VJ Lynch - 《Theory Biosci...