Both microarray-based genotyping analysis for copy number variations and high-throughput next-generation sequencing (NGS) for sequence variations have enabled a surge in the discovery of genes associated with disease. Application of these technologies in large population studies has shown the extent of ...
Following successful sequencing of the human and mouse genomes, a major goal of the genomics community has been to determine the functions of the∼20 000 mammalian protein-coding genes by generating and examining phenotypes of knockout (KO) mice for each gene. Initial efforts towards this goa...
Purified DNA was quantitated using NanoDrop (Invitrogen Life Technologies) and Qubit (Invitrogen Life Technologies). Next-generation sequencing Ion Torrent Ion AmpliSeq Cancer Hotspot Panel v2 (Life Technologies) was used to sequence hotspot regions in 50 frequently mutated tumor suppressor genes and ...
Whole genome wide expression profiles of Vitis amurensis grape responding to downy mildew by using Solexa sequencing technology. BMC Plant Biol. 2010;10(1):1. 62. Laudert D, Pfannschmidt U, Lottspeich F, HollanderCzytko H, Weiler EW. Cloning, molecular and functional characterization of ...
For sequencing, DNA was extracted using AquaStool and cleaned using ethanol precipitation. Microbial composition was assessed using Illumina’s established 16S rRNA amplicon sequencing method and the MiSeq sequencing platform. Briefly, a segment of the V3 and V4 region of the 16S gene was amplified...
Biological systems centering on nucleic acids or encoded proteins greatly benefit from such high-throughput studies, given that large DNA variant pools can be synthesized and DNA, or RNA products of transcription, can be easily analyzed by deep sequencing. Here we review the scope of various high...
Purified DNA was quantitated using NanoDrop (Invitrogen Life Technologies) and Qubit (Invitrogen Life Technologies). Next-generation sequencing Ion Torrent Ion AmpliSeq Cancer Hotspot Panel v2 (Life Technologies) was used to sequence hotspot regions in 50 frequently mutated tumor suppressor genes and ...
Status epilepticus (SE) is a life-threatening condition that can give rise to a number of neurological disorders, including learning deficits, depression and epilepsy. Many of the effects of SE appear to be mediated by alterations in gene expression. To
An array of next-generation sequencing strategies, varying in read length range and absolute throughput [27] can be used to sequence transcriptomes. The Roche 454-pyrosequencing technology, in spite of being the oldest among these, is the one producing on average the longest reads [23, 28, 29...
RAD and double-digest RAD ddRAD [22] sequencing of pooled populations, whole genome sequencing of populations with small genomes such as viruses and mitochondria, targeted-amplicon DNA capture of tumors and populations, and high-throughput rare mutation screens such as the TILLING by sequencing meth...