patients tested had normal Ab responses to diphtheria toxoid, and postimmunization antitetanus titers were abnormally low in five of the 10 patients tested... KA Sheerin,RH Buckley - 《Journal of Allergy & Clinical Immunology》 被引量: 378发表: 1991年 The immune response to hepatitis B vaccine...
and cells with a secretion rate ≥ 3 IgG/s were defined as IgG-SCs. The strength of the interaction between the secreted IgG and the antigen was determined by the slope when plotting the relocation signal of the IgG probe against the relocation signal of the fluorescently labeled TT (SI...
The observation that multiple B-cell subsets have reactivity to VP6 at steady state contrasts the B-cell response to tetanus toxoid39 or to VP7,9 for which reactivity is present solely among switched MBCs. The broad VP6-binding reactivity may represent a regulated mucosal response to a non-neut...
hydrophobic CDR-H2 and has been implicated in early viral responses and polyspecificity.38 The observation that multiple B-cell subsets have reactivity to VP6 at steady state contrasts the B-cell response to tetanus toxoid39 or to VP7,9 for which reactivity is present solely among switched MBCs...
Production of recombinant proteins Recombinant Gag and MinTT (MinTT contained a fragment of HR2, the MPER, the gp41 transmembrane domain and the tetanus toxoid (TT) at C-terminus16,36) proteins were produced in One- Shot™ BL21(DE3) cells (ThermoFisher Scientific) and extracted from ...
(D) Overview over tetanus/diphtheria toxoid (Td) recall responses of 6 random donors tested either fresh or after 2-day high-density preculture. (E) Dose-response to Td of the strongest responder shown in panel D (blue line). Wilcoxon signed rank test: *P≤ .05. Data are mean ± SD...