High- throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry. Journal of Chromatography B 2009; 877: 2664-2672.M. Stahlman, C.S. Ejsing, K. Tarasov, J. Perman, J. Boren, K. Ekroos, High-throughput shotgun lipidomics by quadrupole time-of-flight mass spectrometry, ...
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Fully automatable two-dimensional hydrophilic interaction liquid chromatography-reversed phase liquid chromatography with online tandem mass spectrometry for shotgun proteomics J. Separ. Sci., 35 (2012), pp. 1755-1763, 10.1002/jssc.201200054 Google Scholar [27] M. Gilar, P. Olivova, A.E. Daly, ...
We used a shotgun proteomic approach rather than a classical gel electrophoresis-based approach because many of the glycoproteins are known to be membrane-associated. For the identification of glycopeptides, off-line LC-MALDI-TOF/TOF was used. LC separation prior to MALDI-TOF/TOF was introduced ...
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In recent years, discovery-based proteomic methods, such as the shotgun method discussed above, have been transformed by significant advancements in instrumentation; key figures of merit, such as sensitivity, duty cycle, mass accuracy, and mass resolution have seen remarkable improvements (44). Altho...