In all cases a blood-test with anti-U1RNP, anti-Sm, anti-Ro, and anti-La analysis was conducted. Clinical data was registered according to the patients' medical history, with special emphasis being placed on renal affection, vascular affection, pulmonary hypertension, arthrtitis-synovitis, ...
Enhancing CRISPR-mediated site-specific transgene insertion efficiency by homology-directed repair (HDR) using high concentrations of double-stranded DNA (dsDNA) with Cas9 target sequences (CTSs) can be toxic to primary cells. Here, we develop single-str
5d). Each cell population was immunomagnetically purified with anti-TCR (BW242) and anti-CD4 antibodies to enrich for transgene integration and to deplete cells with mispaired and endogenous TCRs (Fig. 5d–f and Extended Data Fig. 4). As expected, cells that expressed both 1G4-LYK112 and...
To determine the concentration of antibodies in the LSPR-nanosensor, an antibody curve was carried out ranging from 0.25 μg to 10 μg for anti-S and anti-N proteins, respectively (Additional file1: Fig. S1h-l). The concentration chosen was 0.5 μg for both GNR-S and GNR-N nanosensors...
(base EDM only). At day 20, cells were counted and assessed for intracellular HbF staining. Briefly, the cell samples were fixed, permeabilized and stained with anti-human antibodies for fetal hemoglobin (clone HbF-1; Life Technologies). Remaining cells were collected for gDNA extraction for ...
Brain tumours are the leading cause of cancer-related death in children, and there is no effective treatment. A growing body of evidence points to deregulated epigenetics as a tumour driver, particularly in paediatric cancers as they have relatively few
Anti-Tpo-1 antibodies were positive in 10 patients, anti-centromere antibodies in 26 patients, anti-U1-RNP antibodies in 5 patients, and anti-RNA polymerase III antibodies in 3 patient, respectively. The mean of mRSS was 6.3 points. The mean of RHI was 1.68, which was low as compared ...
To test whether Nme2Cas9 is functional by RNP delivery, we loaded purified, recombinant Nme2Cas9 with in vitro-transcribed sgRNAs targeting three validated sites. Electroporation of the RNP induced editing at each site in HEK293T cells (Figure S3C). To test for editing in cells that are ...
Linder KE, Wen MD, Nowotnik DP, et al. Technetium labeling of monoclonal antibodies with functionalized BATO's: 2.TcCl(DMG)3 CPITC labeling of B72.3 and NP-4 whole antibodies and NP-4 F(ab′)2.Bioconjugate Chem1991;2: 407–414. ...
Additional methods of delivery include the use of packaging the nucleic acids to be delivered into EnGeneIC delivery vehicles (EDVs). These EDVs are specifically delivered to target tissues using bispecific antibodies where one arm of the antibody has specificity for the target tissue and the other...