Briefly, the reaction was initiated by mixing an aliquot of homogenate supernatant with 0.5 mM hypoxanthine, 0.5 mM hydroxylamine and 0.01 U xanthine oxidase in the buffer, containing of 104 mM potassium phosphate, 78 mM sodium borate, and 0.025 mM EDTA (PH 7.0) at 37 ...
Acetonitrile, sodium dihydrogen phosphate, potassium dihydrogen phosphate, orthophosphoric acid (HPLC grade) and ascorbic acid were obtained from Sigma Aldrich, USA. Tablets of ascorbic acid, such as Redoxon (1000 mg, Bayer, Basel, Switzerland) and C-Tamin (500 mg, Vital-Health, Las Vegas, NV,...
NHS Digital. National Child Measurement Programme, England, 2021/22 School Year; NHS Digital: Leeds, UK, 2022. [Google Scholar] Australian Institute of Health and Welfare. Autrlian’s Children Overweight and Obesity; Australian Institute of Health and Welfare: Darlinghurst, Australia, 2022. [Googl...
This immunosensor was composed of potassium ferrocyanide, ganglioside (GM1)-functionalized liposomes, and monoclonal antibodies on the surface of Nafion-supported multi-walled carbon nanotubes. The detection mechanism was proposed by a sandwich-type assay, where the toxin was first coupled with an anti...
Molecules 2016, 21, 847 12 of 18 A suspension of potassium dithiocarbazinate (0.57 mmol, 0.25 g) in water (5 mL) and hydrazine hydrate (1.71 mmol, 0.08 mL) was stirred for 1 h. The color of the reaction changed to green with the evolution of hydrogen sulfide gas (lead acetate ...
ABTS•+ was generated by the reaction of 7 mM ABTS (Sigma-Aldrich Chemical Co., Louis, MO, USA) and 2.45 mM potassium persulfate (Merck Chemicals, Darmstadt, Germany) in PBS (pH: 7.4) in a dark place at room temperature for 16 h. ABTS values are expressed in µmol Trolox (Sigma...