D. Protein phosphorylation in signaling–50 years and counting. Trends Biochem. Sci. 30, 286–290 (2005). Article CAS Google Scholar Salazar, C. & Höfer, T. Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein-protein interactions. FEBS ...
The cells were fixed with 4% paraformaldehyde for 20 min at room temperature and washed with phosphate-buffered saline (PBS). After permeabilizing the membrane and blocking, the cells were incubated with the following primary antibodies at 4°C overnight: rabbit anti-STAR (12225–1-AP; ...
Comprising K 2 HPO 4 , 12.54; KH 2 PO 4 , 2.31; D-Glucose, 4.0; NH 4 Cl, 1.0; Yeast extract, 12.0; NaCl, 5.0; MgSO 4 , 0.24; the medium possessed a high capacity phosphate buffer able to moderate pH fluctuations during cell growth known to be detrimental to biomass formation. ...
High Quality Tech Grade Sodium Tripoly Phosphate CAS No. 7758-29-4 (W~ha~ts~Ap`p+86 18~56~1~73`~52~6`5) --Basic Info.-- Chemical Name:Sodium tripolyphosphate CAS No.:7758-29-4 Molecular Formula:Na5P3O10 Molecular Weight:367.86 EINECS No.:231-8...
sphingosine-1-phosphate Abbreviations AdipoR1/2 adiponectin receptor1/2 AMPK AMP-activated protein kinase BAT brown adipose tissue BSA bovine serum albumin HFD high-fat diet ITT insulin tolerance test OGTT oral glucose tolerance test PA palmitic acid PEG polyethyleneglycol PI propidium iodide PPAR perox...
All standards, controls and samples were diluted in 10 mM sodium phosphate buffer pH 7.2, 150 mM sodium chloride, 0.7% triton X-100, and 0.1% sodium azide containing 1% BSA. After a 2-h incubation, the wells were washed thrice with 400 μl of 1x PBST using a plate washer. The ...
Glycerate-3-phosphate, produced by CO2 fixation in the Calvin cycle, is critical for the synthesis of the D1 protein of photosystem II. We demonstrated recently that, in intact cells of Chlamydomonas reinhardtii, interruption of CO 2 fixation via the Calvin cycle inhibits the synthesis of p.....
Magnesium (Mg2+) is an essential nutrient in all organisms. However, high levels of Mg2+ in the environment are toxic to plants. In this study, we identified the vacuolar-type H+-pyrophosphatase, AVP1, as a critical enzyme for optimal plant growth under
D. Protein phosphorylation in signaling–50 years and counting. Trends Biochem. Sci. 30, 286–290 (2005). Article CAS Google Scholar Salazar, C. & Höfer, T. Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein-protein interactions. FEBS ...