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After 1 h of saturation (Intercept [TBS] Blocking Buffer, LI-COR, Lincoln, NE, USA) at room temperature, membranes were incubated overnight at 4°C with anti-mouse InsR antibody diluted 1/2000 (sc-57342; Santa Cruz Biotechnology, Santa Cruz, CA, USA [24, 25]) and anti-rabbit GAPDH...
6-Lincoln Sledzianowski (Bishop McCort, PA) SO 7-Antonio Rodriguez (Los Gatos, CA) JR 8-Paul Kenny (Christian Brothers Academy, NJ) SO 9-Gavin Jendreas (Crown Point, IN) SR 10-Edwin Sierra (Poway, CA) SR 11-Haakon Peterson (Hollondale, WI) JR 12-Sebastian Dege...
Membranes was rinsed again in TBS-T, followed by TBS, incubated in SuperSignal West Pico PLUS (Thermo Fisher, 34578), and developed on a Li-Cor C-Digit Blot Scanner using Image Studio software (v.5.2 Li-Cor Biosciences, Lincoln, NE, USA). Following developing, the membrane was rinsed in...
Total absorbed light was also calculated by combining the percentage of actinic light emitted by the blue (470 nm) and red (640 nm) LEDs in the leaf gas exchange chamber with α for the peak wavelength of the two LED types36. The apparent maximum qCuOa2nmtuomleyciuelleds(aΦb ...
University of Lincoln, Lincoln, UK James Tonks Haven Clinical Psychology Practice, Cornwall, UK James Tonks Biomedical Engineering Laboratory, Department of Telecommunication and Control, Avenida Professor Luciano Gualberto, Travessa 3, n. 158. Cidade Universitária, São Paulo, SP, 05508-010, Brazil...
Department of Agricultural Economics, University of Nebraska-Lincoln, Lincoln, NE, USA Taro Mieno & Nicholas Brozović School of Engineering, University of Manchester, Manchester, UK Timothy Foster Department of Applied Economics, University of Minnesota, Minneapolis, MN, USA Shunkei Kakimoto Daugherty...
The best days of disco: New York nightlife by Bill Bernstein in pictures - "From Studio 54 to Paradise Garage, Bernstein was there to document the euphoric late 70s peak of the New York City disco scene." The world’s best private members clubs - ShortList Magazine. THE WORLD'S FINEST...
After 30 min at RT, once the Click reaction was complete, the wells were aspirated, and 40 µl of Odyssey blocking buffer (catalog number 927-40150, LI-COR Biosciences, Lincoln, NE) was added per well for a minimum of one hour at RT, or up to overnight at 4 °C to ...
{3}d\)) with a lattice parameter of 12.93 Å. The peak of the pristine pellet is clearly asymmetric due to the presence of the tetragonal phase. (Details of the Rietveld refinement are provided in Supplementary Figs.18and21, and details of the refinement approaches are described in ...