The 1,000-4,000-bp DNA fragments were successfully generated by PCR and 5-, 6-, 8- and 10-kb DNA fragments were obtained through the digestion of the plasmids. The bands of the prepared high mw DNA ladder were clear and may aid future molecular biology studies.Jun-He...
DescriptionGeneRuler High Range DNA Ladder is recommended for fast sizing and approximate quantification of high molecular weight double-stranded DNA on agarose gels (in 1.5 hour, 0.4% agarose gel). The ladder is a mixture of chromatography-purified individual DNA fragments. GeneRuler High Range DNA...
The same targets were also amplified using competitor DNA polymerases: A: Merck KOD™ Hot Start, B: KAPA HiFi HotStart PCR Kit, C: PrimeSTAR GXL. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder. PCR of different templates with Platinum SuperFi II DNA Polymerase...
The same targets were also amplified using competitor DNA polymerases: A: Merck KOD™ Hot Start, B: KAPA HiFi HotStart PCR Kit, C: PrimeSTAR GXL. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder. PCR of different templates with Platinum SuperFi II DNA Polymerase...
PacBio HiFi sequencing provides highly accurate long-read sequencing datasets which are of great advantage for whole genome sequencing projects. One limitation of the method is the requirement for high quality, high molecular weight input DNA. This can b
High molecular weight hyaluronan decreases UVB-induced apoptosis and inflammation in human epithelial corneal cells. Mol. Vis. 15, 577–583 (2009). CAS PubMed PubMed Central Google Scholar Takasugi, M. et al. Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective ...
Genetic markers are crucial for breeding crops with desired agronomic traits, and their development can be expedited using next-generation sequencing (NGS) and bioinformatics tools. Numerous tools have been developed to design molecular markers, enhancin
40 30 20 10 High molecular weight DNA is consistently extracted from samples collected with OMNIgene•GUT Due to the competitive nature of PCR, DNA degradation in microbiome samples can lead to the introduction of bias since smaller DNA fragments may be preferentially amplified. Therefore, ...
First, the self-assembling and amplified DNA duplex formed by HCR can provide many binding sites for molecular dug via the groove binding or covalent modification.61 Wang et al.22 conjugated a synthesized prodrug into the terminal of H2. The activation of HCR can cause the accumulation of ...
In addition, extraction of high-quality RNA from plant tissues subjected to different environmental stresses such as heat, drought and cold stresses can be difficult due to increased accumulation of high molecular weight polysaccharides and secondary metabolites that interfere with RNA isolation. Secondary...