At the prevalences found in the present study, the predictive value of early high IgG4 Ab level for treatment failure was 0.6, whereas the predictive value of early low IgG4 Ab level for treatment success was 1.00. 展开 关键词: IMMUNOTHERAPY ALLERGENS QUANTITATIVE research THERAPEUTICS CLINICAL ...
Luxembourg Inst Hlth Dept Infect &VaccineHubschen, J.M.; Charpentier, E.; Weicherding, P.; Muller, C.P. IgG antibody prevalence suggests high immunization needs in newcomers to Luxembourg, 2012. Vaccine 2018, 36, 899-905. [CrossRef] [PubMed]...
Methods . The turbidity and the association state of an IgG1 antibody were investigated as a function of concentration and temperature using static and dynamic light scattering, nephelometric turbidity, and analytical ultracentrifugation. Results . The antibody had increasingly opalescent appearance in the...
Treatment of B16F10-challenged wild-type mice with TA99 antibody specific for the gp75 tumor antigen resulted in a marked decrease in numbers of lung metastases. Treatment of individual FcgammaR knock-out mice revealed the high-affinity IgG receptor, FcgammaRI (CD64), to represent the central...
Protein spots were assessed for quality, circularity, and consistency using mouse monoclonal anti-tetra-histidine IgG antibody (Qiagen) diluted 1:2000 in TBS (20 mM TrisHCl, 150 mM NaCl, pH 7.6) to detect hexa-histidine-tagged antigens. An anti-mouse IgG antibody (Sigma) diluted 1:5000...
Primary antibody labeling was performed at 4 °C overnight with a mix of two anti-α-tubulin mouse monoclonal IgG1 antibodies (DM1A (T6199, Sigma) and B-5-1-2 (T5168, Sigma)) diluted 1:300 in blocking buffer. After 3 × 10-min washes with blocking buffer, the cells were in...
特异性anti- Rat IL-6 capture antibody预包被在高亲和力的酶标板上。酶标板孔中依次加入标准品和待测样本,充分振荡混匀后置于室温下进行为时2小时的孵育过程,样本中存在的IL-6与固相抗体结合。充分洗涤以除去游离及未结合的成分后,加入生物素化的检测抗体孵育。再次洗涤后,加入辣根过氧化物酶标记的链霉亲和素(...
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Background: Hemolytic disease of the fetus and the newborn (HDFN) is the consequence of the destruction of the fetal and neonatal red blood cells by IgG antibodies acquired by the transplacental route from the mother. After the introduction of Rh- immune globulin in 1968, the incidence of ...
Sonicated samples were spun down and subjected to overnight immunoprecipitation with IgG or FoxM1 antibody (Santa Cruz Biotechnology). After the proteins and RNA are removed by Protease K and RNAase A, the chromatin pulled-down by antibodies is purified. The enrichment of STMN1 is detected by ...