Generation and characterization of high affinity humanized fab against hep- atitis B surface antigen. Mol Biotechnol. 2009;43(1):29-40. doi:10.1007/s12033-009-9165-9.Ashutosh Tiwari,Durgashree Dutta,Navin Khanna,Subrat K. Acharya,Subrata Sinha.Generation and Characterization of High Affinity ...
Taken together, the hYP7 antibody is more potent than hYP9.1b in both ADCC and CDC assays. Therefore, the hYP7 antibody was selected for mouse testing. Evaluation of hYP7 in mice. To determine the effect of hYP7 on in vivo cell killing, we used a Hep3B HCC xenograft tumor model ...
(a) Overexpression of GNA13 could not promote the proliferation of HepG2 and SMMC-7721 cells in vitro as determined by MTT assay. (b) In the invasion assay, the cell invasion ability was significantly increased after overexpression of GNA13 in HepG2 and SMMC-7721 cells when compared with ...
The human liver cell line (HepG2) did not have any expression for Epac1 gene (A, lane 2). The HK-2 cells revealed a ∼4 Kb transcript (B, arrowhead) and a protein product of ∼90 kDa size (C, arrowhead), by Northern and Western blot analyses, respectively; thus indicating a ...
Selective stimulation of murine cytotoxic T cell and antibody responses by particulate or monomeric hepatitis B virus surface (S) antigen In the murine system, we tested in vivo the immunogenicity of different preparations of the yeast-derived surface antigen (S-antigen or S-protein) of hep... ...
To determine immunereactivity, resolved proteins were transferred to a nitrocellulose membrane and immunoblotted with rabbit IgG anti-peanut agglutinin antibody (1:1000). Biodetection was determined with chromogenic peroxidase substrate-based detection of HRP activity. (A) Immunoblotting results of total ...
Here we report that HDL was detected at the cell surface in clathrin-coated invaginations using DAB staining and electron microscopy (Figure 2), indicative clathrin-mediated endocytosis. Indeed, using HepG2 cells, we saw a similar binding and uptake behavior of HDL, with the involvement of endo...
Hep par 1 antibody stain for the differential diagnosis of hepato- cellular carcinoma: 676 tumors tested using tissue microarrays and conventional tissue sections. Mod Pathol 2003;16:137–144. 40 Lee MJ, Vogt AP, Hsiao W et al. CDX-2 expression in malignant germ cell tumors of the testis,...
we therefore carried out IHC staining of CK7 and CK9 in liver tissue/tumor sections derived from the mice implanted with either WBCtrlor WBBMI1cells, indicating the presence of CK7 and CK19 positive bile duct epithelial fragments on the surface of the tumor thrombi in WBBMI1implanted mice...
"Genetically Engineered Diphtheria Toxin Fusion Proteins Carrying The Hepatitis B Surface Antigen", Gene 55:255-263 (1987). Zucker et al. "Monoclonal Antibody Analysis of Diphtheria Toxin--I. Localization Of Epitopes And Neutralization Of Cytotoxicity", Molecular Immunology 21(9):785-793 (1984)....