The primers are: Rat: Bcl-2 forward: GGATCCAGGATAACGGAGGC, Bcl-2 reverse: ATGCACCCAGAGTGATGCAG; GAPDH forward: AGTGCCAGCCTCGTCTCATA, GAPDH reverse: TGAGGTCAATGAAGGGGTCG; U6 snRNA forward: CTTTGTAGGCTTCAGCGGAG, U6 snRNA reverse: ATGACGTCTGCCTTGGAGAAC. Human: Bcl-2 forward: GGGGAG...
Each breed was compared with other eight lowland breeds found in Asia based on the results of the Ggtiecenuneleatpricloypswt4re.u3llcsstouufirttewe.daTrfeho4re6.duTenhtebecinatistnhegdeldienissettiaamgteias-ttseipcoeacftipfeiaaccisrhewlSeiNcsetPivFmeSTeavraeksnedrtsef,oswcrraeisabccehadlhcb...
In the neutralization assay, although the addition of DEAE-dextran could reduce the virus volume to yield similar levels of SNR, this had little effect on the final results. Based on these observations, 10 μg/ml of DEAE-dextran was chosen to enhance the infectiousness of the ...
GAPDH GGTCGGAGTGAACGGATTT CTCGCTCCTGGAAGATGG Note. BMP-2, bone morphogenenetic protein-2; OPN, osteopontin; TGF-β1, transforming growth factor-β1; RANKL, receptor activator of nuclear factor κB ligand; OPG, osteoprotegerin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. | Show Table DownLoa...
Bsui (B. suis) F-TGCGCTATGATCTGGTTACGTTR-AGCGCGGTTTTCTGAAGGT Transposase 69 Boa (B. abortus and B. ovis) F-GACCTCTTCGCCACCTATCTGGR-CCTTGTGCGGGGCCTTGTCCT glk 163 The HRM curves of the primer sets were then normalized using 2 normalized regions of the premelt phase (melting phase of ...
The primers are: Rat: Bcl-2 forward: GGATCCAGGATAACGGAGGC, Bcl-2 reverse: ATGC ACCCAGAGTGATGCAG; GAPDH forward: AGTGCC AGCCTCGTCTCATA, GAPDH reverse: TGAGGTCA ATGAAGGGGTCG; U6 snRNA forward: CTTTGTAG GCTTCAGCGGAG, U6 snRNA reverse: ATGACGTC TGCCTTGGAGAAC. Human: Bcl-2 forward: ...
Table 1 Primers sequences used for quantification of gene expression by real-time PCR and the resulting product size Gene Primers Size (bp) Renin Angiotensinogen ACE 18S rRNA PPARa 5¢-TTCTCTCCCAGAGGGTGCTA-3¢ 5¢-CCCTCCTCACACAACAAGGT-3¢ 5¢-CACGGACAGCACCCTATTTT-3¢ 5¢-G...
(Takara, RR037). Two pairs of primers were designed to detect the Homo sapiens endothelial PAS domain protein 1 (EPAS1) (NM_ NM_001430.5). The primer sequences were as follows, F: 5’-TCCACCTTCAAGACAAGGTCTG-3’, R: 5’-GTACATTTGCGCTCAGTGGC-3’; and GAPDH, F: 5’-CGGAGTCAACG...
Electroacupuncture pretreatment at PC6 acupoints, but not at non-acupoints, decreased the number of HMGB1-positive cells in ischemia-reperfusion animals, although it remained higher than the number in control animals (Fig. 2E). After 24 h of reperfusion (time point R24), levels of HMGB1 ...
The following specific primers (5′→3′) were chosen for analysis: for the alpha-toxin Cpa gene, F: GCTAATGTTACTGCCGCCGTTGA, R: CCTCTGATACATCGTGTAAGAATC, for the cpb beta-toxin gene—F: GCGAATGCTGAATCATCTA, R: GCAGGAACATTAGTATATCTTC, for the etx epsilon-toxin gene—F: GCGGT...