Plant transformation is an invaluable tool for basic plant research, as well as a useful technique for the direct improvement of commercial crops. Barley (Hordeum vulgare) is the fourth most abundant cereal crop in the world. It also provides a useful mo
Amplification of BiTE gene from the cDNA using BiTE gene-specific forward PCR primer annealing to signal sequence (5’-GCTGGTCCTGCATCATCCTGTTTCTG-3’) annealing to the signal sequence and reverse PCR primer annealing to the 2A sequence (5’-CCGGGGTTTTCTTCCACGTCTCC-3’). A second nested ...
This latter result implies the cell population observed can be measured at a level of 380 cells (4% of ~9500). Individual cells can be clearly observed, meaning the definition rate of cells which have sufficiently unique markers can be observed at the rate of 1 in 9500 or 0.01%. Note th...
Nevertheless, the putative meaning of some of the other mutations can carefully be extrapolated from other flaviviruses. Three out of six of our identified amino acid exchanges occurred within a fragment reaching from position 305 to 346. In this part of domain III, mutations have been reported ...
H. GGTDB-Tk v2: memory friendly classification with the genome taxonomy database. Bioinformatics 38, 5315–5316 (2022). 36. Louca, S. & Doebeli, M. Efficient comparative phylogenetics on large trees. Bioinformatics 34, 1053–1055 (2018). 37. Yu, G. Using ggtree to visualize data on ...
12 After isolation, total DNA was extracted, diluted to 10 ng μl−1 and quantitative real-time PCR was performed using 16s rRNA primers: 5′-GTGSTGCAYGGYTGTCGTCA-3′; 5′-ACGTCRTCCMCACCTTCCTC-3′; Firmicutes primers: 5′-GGAGYATGTGGTTTAATTCGA-3′; 5′-AGCTGACGACAACCATGCAC...
GGGGACAAGTTTGTACAAAAAAG CAGGCTTGGTGCCACGCGGCAGC, and R2 contains attB2 and the termination codon: GGGGACCACTTTGTACAAGAAAGCTGGGTTA. Gene specific regions in F1 and R1 are designed by BatchPrimer that would result in a pair of primers with a similar melting temperature (Tm) by adjusting th...
(5′-CCGGTCTGAACTCAGATCACGT-3′) (Palumbi1994). PCR was performed in a total volume of 25 µl including, 1U of DreamTaq polymerase, 0.2 µM of each dNTP, 1× DreamTaq buffer, 0.2 µM of forward (16SbrH(32)) and reverse (16Sar(34)) primers and 4 µl of crushed ...
The position of the cells on the graph has no physical meaning and was arbitrarily adjusted to group all ideal clonotypes on one side. Acknowledgments This work was made possible by the support from the Beijing Advanced Innovation Center for Genomics (ICG) at Peking University and the ...
The level in the present composition of cellulase described above should be such that the amount of enzyme protein to be delivered in the wash solution is from 0.005 to 40 mg/liter of wash solution, preferably 0.01 to 10 mg/liter of wash solution. ...