Borderline high serum free light chain k/ ratios are seen not only in dialysis patients but also in non-dialysis-dependent renal impairment and inflammatory states. Am J Clin Pathol 2009; 132: 309.Marshall G, Tate J, Mollee P. Borderline high serum free light chain kappa/lambda ratios are...
The TdT-tailed cDNA was PCR amplified using the same 5′ anchor primer as for the heavy chain variable region, plus a kappa chain nested 3′ primer (5′-CTCATTCCTGTTGAAGCTCTTGACAATGGG-3′). The product of the reaction was purified using a NucleoSpin gel and PCR clean-up kit and ...
Sequences were codon-optimized and cloned into separate vectors containing the human IgG1 or human kappa constant regions, respectively. Plasmids were transiently transfected in HEK293 cells and the antibodies provided as harvested cell culture fluid (Twist Bioscience). Some monoclonal antibodies were ...
tssViuunLlbgtgsi1nee4qngueppseaa,nii4rrtsl8eydwrseeVesrquHeul/ctVeelnodLcngieeneddnrveieniscatofooSvarhenrerygeapveorylficsaVeanqtHdeugsleieengnqhceuitnsecgnoh.cnaOeliysnf,, mammalian expression plasmids containing human IgG1 constant regions and kappa light chain regions for chimeric ...
Hence, the anti-inflflammatory activity of lupeol could be explained by the suppression of NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) expression in keratinocytes and the regulation of MMP-2 (matrix metalloproteinase-2) [42...
Shop Kappa Diadora Diadora1 / 2 Stepping away from Italian luxury brands, Diadora stands with the giants in the classic sportswear and sneaker arena. Founded in 1948, the Venetian brand’s first product was a pair of mountain boots and soon transitioned to the football world with the help of...
A single cell-based 5′ RACE PCR of these R4-gated cells resulted in the amplification of cognate pairs of VH and light chain kappa variable (VL) genes. DNA transfection of pairs of immunoglobulin heavy (IgH) and immunoglobulin light (IgL) genes into 293 cells resulted in the production of...
Antibodies Primary antibodies against Akt (clone C67E7; #4691), phosphorylated Akt (P-Akt; phosphorylation on serine 473; clone D9E; #4060), nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha (IkB-α; #9242), JNK (c-Jun N-terminal; #9252), phosphorylated...
Formed by two equal LT mirrors (see Sect. 2), the rigid FFPC has the following cavity parameters at 850 nm wavelength: cavity length \(L_\mathrm{{cav}} = 47.96\pm 0.01\,\upmu{\text{m}} \), finesse \(\mathcal {F}=19{,}000\pm 2000\), cavity linewidth \(\kappa = 2\pi \...
A single cell-based 5′ RACE PCR of these R4-gated cells resulted in the amplification of cognate pairs of VH and light chain kappa variable (VL) genes. DNA transfection of pairs of immunoglobulin heavy (IgH) and immunoglobulin light (IgL) genes into 293 cells resulted in the production of...