in situ signal detection and decoding, and cell classification. First, for all channels except DAPI, the background images were subtracted from the original images to decrease autofluorescence of cytoplasm and other objects such as erythrocytes, thereby...
16). Except for erythrocytes, which have strong characteristic spectra and low heterogeneity, sensitivity was overall around 65%. Hence, Raman spectroscopy has a good potential to exclude the existence of specific cell types in a scan, but cannot securely define if these occur in the scan. Thus...
Fe2+-heme, it was imperative to measure the Kd values for the heme·FLRev-erbβ complex to determine whether the full-length proteins could bind heme at the low intracellular concentrations of free/exchangeable heme, which has been estimated to be <0.2 μm in mature erythrocytes (78, 79)....
While marginally increased levels of ADCD were observed using serum samples, these differences were not statistically significant (Fig. 6A). Moreover, C3 deposition results for serum and plasma samples correlated highly, suggesting that both serum and plasma can be used in the ADCD assay (Fig. ...
S. aureusis the human pathogen that displays the widest assortment of virulence factors [73]. Beta-hemolysin, beta-toxin or sphingomyelinase C, one of the many exotoxins secreted byS. aureus, is a sphingomyelin phosphodiesterase (EC 3.1.4.12) that disrupts the membranes of erythrocytes and...
Elevated levels of AA, a PUFA which is a marked substrate for the formation of MDA via lipid peroxidation, were found in the erythrocyte membrane in patients with psoriasis [114]. Similarly, the MDA content in erythrocytes of those patients was increased as well [115]. Furthermore, ROS ...
In the twenty-first century, the terms “cosmetics” and “cosmetology”, meaning “the art of body care”, refer to not only a wide range of products and application techniques but also a multisector industry for which modern medical laboratories work, exclusively focusing on the beautifying as...