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Coding sequences were compared with the human genome (GenBank™ build 25) using BLAST, and the exons were extracted and set in-frame. For ORFs containing multiple exon, the first was discarded to reduce the likelihood of a signal peptide, and from the remaining exons the longest was ...
The town headed off the attempt to build another mall.Idioms: have a big/swelled head To be overly self-confident or conceited. head and shoulders above Far superior to: head and shoulders above her colleagues in analytical capability. head over heels 1. Rolling, as in a somersault: ...
1 bead ratio to eliminate the presence of primer dimers. The qualities of the libraries were determined on the Agilent Bioanalyzer using a DNA high sensitivity chip and the concentrations were determined using the Invitrogen Qubit fluorometer. The barcoded samples were then pooled and sequenced on ...
The promoter of Hsp17.6 was used to build a Hsp17.6p:GFP transcriptional reporter that is induced by heat shock in Arabidopsis. An automated fluorescence microscopy system which enables multiple roots to be imaged in rapid succession was used to quantitate Hsp17.6p:GFP response dynamics. Hsp17.6p...
The primers used to introduce these point mutations were designed using the NEBaseChanger interactive tool from New England Biolabs. All constructs were analysed through DNA sequencing to confirm the presence of the desired point mutations and to verify that no additional mutations were generated. ...
Get technical advice Download data sheet Select Language English A high temp metal that can take the heat If you need to build heavy equipment that can fight wear in tough, high-temperature applications, take a look at Hardox®HiTemp wear resistant steel. While traditional Q&T wear-resistant...
the electrically conductive coating provides grounding means for the HDD and is capable of shielding the HDD from electromagnetic interference (EMI). Also advantageously, the electrically conductive coating prevents the build up of static charges on the surface of the coated HDD base. This helps to...
However, this technique, although widely used in academic research and clinical diagnosis, has several limitations, such as the technical detection of the target protein, its precise quantification, the duration of the experiment and the costs of the reagents. Altogether, these limitations preclude ...
Table 1. Primers sequences that were used for quantitative PCR. 2.9. Statistical Analysis A total of three experiments were carried out and each experiment included four parallel samples for group (n = 4). The data are reported as the mean ± the standard deviation (SD). The different gr...