Among 357 patients who developed PNALD, the highest direct bilirubin concentrations correlated with the highest serum alkaline phosphatase and transaminase concentrations. Deaths after 28 days were much more common in those with the highest direct bilirubin and transaminase concentrations (P<0.0001). ...
Escherichia coli alkaline phosphatase (AP) can hydrolyze a variety of chemically diverse phosphate monoesters while making contacts solely to the transferr... LD Andrews,H Deng,D Herschlag - PubMed 被引量: 19发表: 2009年 Effect of high ammonia loads emitted from poultry-manure digestion on nitr...
42. To identify transcriptional interactions underlying hypoxia acclimatization, we used thek-means method to analyze multi-tissue interactions based on log2FC (log2-transformed fold change) values in the
Alkaline phosphatase-conjugated goat anti-rabbit IgG (Santa Cruz) was used as secondary antibody (1:5000 dilution), and immunoreactive bands were detected with ECL solution (Thermo Scientific), according to the manufacturer’s instructions. Quantification and statistical analysis Data extraction and ...
Forward genetic screens in mice provide an unbiased means to identify genes and other functional genetic elements in the genome. Previously, a large scale ENU mutagenesis screen was conducted to query the functional content of a ~50 Mb region of the mous
Automatic binning was attempted by means of Metawatt 3.276. Four high quality, four medium quality, and 21 partial bins were generated. These bins were further polished manually by eliminating contigs assigned to different phyla within a bin, or with widely different sequence coverage. Three bins ...
(2018) established a high-throughput screening model at the cellular level using secretory embryonic alkaline phosphatase (SEPA) as a reporter gene on 384-well plates, and 25 positive compounds were identified from 32,000 compounds. The cytotoxicity, phosphorylation effect on STAT protein a...
Intensity of the colorimetric output of the antibody-bound alkaline phosphatase reactions (panelsa(NC),c(N),e(V),g(P) andi(P-18)) and a structural representation of each mapped region on the crystal structure of VP2 for each condition (panelsb(NC),d(N),f(V),h(P) andj(P-18))....
elevated CRP levels have been associated with higher alkaline phosphatase and alanine aminotransferase activities in metabolic syndrome subjects. The magnitude of elevated CRP observed at fasting and postprandial in pro-atherogenic subjects could therefore indirectly point to elevated liver function26,27. A...
For radioactive labeling, 50 pmol of the in vitro transcript were dephosphorylated using 25 U of calf intestine alkaline phosphatase (NEB) in a 50 µL reaction volume and incubated for 1 h at 37 °C. Following extraction with phenol:cholorform:isoamylalcohol (P:C:I, 25:24:1),...