For radioactive labeling, 50 pmol of the in vitro transcript were dephosphorylated using 25 U of calf intestine alkaline phosphatase (NEB) in a 50 µL reaction volume and incubated for 1 h at 37 °C. Following extraction with phenol:cholorform:isoamylalcohol (P:C:I, 25:24:1),...
Long-term exposure to hyperglycemic conditions leads to β-cell dysfunction, particularly mitochondrial dysfunction, and inflammatory and oxidative stress responses, which are considered the primary causes of β-cell death and the hallmarks of diabetes.
Laboratory tests included complete blood count, total protein, albumin, calcium, inorganic phosphorus, fasting plasma glucose, blood urea nitrogen, uric acid, total bilirubin, alkaline phosphatase, ALT, AST, gamma glutamyl transpeptidase, serum creatinine, thyroid stimulating hormone (baseline only), Hb...
Use of progesterone 11-glucuronide-alkaline phosphatase conjugate in a sensitive microtitre-plate enzyme-immunoassay of progesterone in milk and its application to pregnancy testing in dairy cattle J. Reprod. Fertil., 76 (1986), pp. 375-391 Google Scholar Schauff and Clark, 1992 D.J. Schauff,...
(e.g., enzymes such as horseradish peroxidase, alkaline phosphatase, etc.) or through the use of a specific binding molecule which itself may be detectable (e.g., a labeled antibody that binds to the second antibody, biotin, digoxigenin, maltose, oligohistidine, 2,4-dintrobenzene, phenyl...
Nissen SE, Nicholls SJ, Sipahi I, Libby P, Raichlen JS, Ballantyne CM, Davignon J, Erbel R, Fruchart JC, Tardif JC, etal: Effect of very high-intensity statin therapy on regression of coronary atherosclerosis: the ASTEROID trial. JAMA. 2006, 295 (13): 1556-1565. 10.1001/jama.295.13....
Age years old, Hei height, Wei weight, BMI body mass index, NCE neck circumference, ACE abdominal circumference, WSBP systolic blood pressure, WDP diastolic blood pressure, CHO cholinesterase, ALB albumin, GLO globulin, γ-GPE γ-glutamyl transpeptidase, ALK alkaline phosphatase, AST aspartate ...
Plates were developed by incubation with anti-IFNγ–biotin and streptavidin-alkaline phosphatase according to the manufacturer’s instructions (Mabtech). Spots were developed using the AP Conjugate Substrate kit (Bio-Rad). Spots were counted in an automated ELISpot reader (C.T.L. ImmunoSpot). ...
Significant decreases in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bile acids (TBA), alkaline phosphatase (ALP), and direct bilirubin (DBIL) levels were observed in ZTHL-group versus the CH-group (p < 0.05). Serum alpha-L-fucosidase (AFU) levels in ...
This coincided with a significant reduction in systolic blood pressure (SBP) from a baseline value of 123 ± 15 mmHg up to 119 ± 14 mmHg (p = 0.019) and a reduction in alkaline phosphatase (213 ± 55 mg/dL to 202 ± 55 mg/dL, p = 0.012). The vitamin D-treated group showed no...