6. Scull, C.E., Dandpat, S.S., Romero, R.A., and Walter, N.G. (2020). Transcriptional riboswitches integrate timescales for bacterial gene expression control.Front. Mol. Biosci.7, 607158. 转载须知 【原创文章】BioArt原创文章,欢迎个人转发分享,未经允许禁止转载,所刊登的所有作品的著作权均...
Lactobacillus casei suppresses hfq gene expression in Escherichia coli O157:H7Escherichia coli O157:H7Lactobacillus caseihfq geneQ-PCRdoi:10.1080/09674845.2019.1567903S MahdaviA IsazadehBritish journal of biomedical science
1.1.3实验材料 2X HiFi Master Mix、聚合酶链反应(PCR)产物回收磁珠购自Biorise Life Science公司;质粒pTargetF、pBBR1MCS-2、pCas9购自Addgene公司,其中pTargetF (Cm)质粒为本实验室保存,在pTargetF的664～1 323位点间加入了氯霉素抗性基因;质粒pSMART-LCKan购自Lucigen公司;Kpn I-HF、HindⅢ-HF、Turbo感受态...
Hfq is an RNA-binding protein that is common to diverse bacterial lineages and has key roles in the control of gene expression. By facilitating the pairing of small RNAs with their target mRNAs, Hfq affects the translation and turnover rates of specific transcripts and contributes to complex pos...
[Key words] Salmonella enterica serovar Typhi; hfq; hyperosmotic stress; gene microarray 伤寒沙门菌(Salmonella enterica serovar Typhi,)是一种人类严重的肠道致 病菌,已成为一种重要的原核基因表达与调 控研究的模式生物。hfq 基因编码的蛋白是 一种分子伴侣,最早是作为大肠埃希菌 RNA 噬菌体 Qβ 复制所必需...
摘要：非编码小ＲＮＡ（ｓｍａｌｌＲＮＡ，ｓＲＮＡ）是细菌基因转录后调控的一个重要层次，也是近十年来原核生物研究领域的焦点之一㊂大多数ｓＲＮＡ的作用与Ｈｆｑ蛋白密切相关，即Ｈｆｑ可以促进ｓＲＮＡ与其靶标ｍＲＮＡ的互补配对，进而影响翻译的进行或者ｍＲＮＡ的稳定性㊂笔者对Ｈｆｑ的结构㊁Ｈｆｑ...
Cronobacter sakazakii strain ATCC 29544 putative RNA-binding protein (hfq) gene, complete cds 来自 ResearchGate 喜欢 0 阅读量: 33 作者:S Kim,H Shin,S Ryu 摘要: Assembly-Data-START## Assembly Method :: SeqMan v. 8.1.3 Sequencing Technology :: ABI 3730xl DNA analyzer ##Assembly-Data-END...
SummaryHfq is an RNA-binding protein that interacts with both small untranslated RNAs (sRNAs) and mRNAs to modulate gene expression post-transcriptionally. In Escherichia coli and Salmonella typhimurium, Hfq is required for efficient expression of the stationary phase sigma factor σS, and consequently...
The global regulator Hfq facilitates the action of regulatory RNAs in post-transcription gene regulation in many bacteria. In Pseudomonas aeruginosa , Hfq, in conjunction with the catabolite repression protein Crc, forms a complex that directly inhibits translation of target transcripts during carbon cata...
Specifically, two single-point mutations in RelA (C289Y and T298I) that clustered in one helix of the RelA N-terminal domain failed to enable repression of RyhB target gene fusions. Unlike wild type RelA that facilitated RNA binding of low concentrations of Hfq by triggering Hfq ...