Objective: Aim of the present study is to compare the two automated enzymatic methods to understand and familiarize the principles of method selection and evaluation Materials and Methods: 762 individuals plasma glucose estimation were included in study. Results: Present study the glucose concentration ...
The method below provides a means for obtaining continuous rate measure- ment with lowsubstrate utilization. It is particularly useful for estimation of K~ values. 8 The soluble hexokinaserepresenting about The suspension of particulat...
During the cancerous transformation of normal hepatocytes into hepatocellular carcinoma (HCC), the enzyme catalyzing the first rate-limiting step of glycolysis, namely the glucokinase (GCK), is replaced by the higher affinity isoenzyme, hexokinase 2 (HK2). Here, we show that in HCC tumors the hi...
Hexokinase-3 (HK3), as a member of the hexokinase family, is involved in the first step of glucose metabolism, and its coding gene is located on the human chromosomal 5q35.2 segment. In colorectal cancer cell lines, there is feedback regulation of the expression levels of HK1 and HK2; ...
The applicability of the proposed method has been examined for the closed three-enzyme system of rabbit heart creatine kinase (EC 2.7.3.2), yeast hexokinase (EC 2.7.1.1) and human erythrocyte glucose 6-phosphate dehydrogenase (EC 1.1.1.49) using experimental data....
The applicability of the proposed method has been examined for the closed three-enzyme system of rabbit heart creatine kinase (EC 2.7.3.2), yeast hexokinase (EC 2.7.1.1) and human erythrocyte glucose 6-phosphate dehydrogenase (EC 1.1.1.49) using experimental data....