Metabolic impact (increased glycogen deposition, [5-3H]glucose usage, and lactate production) is also clearly evident when glucokinase is overexpressed in hepatoma cells (28) or primary hepatocytes. 3 These data suggest either that overexpressed glucokinase is inhibited by an islet-specific factor or...
Hexokinase PII is not inhibited by high Mg-ATP concentrations if the Mg2+-free ATP is kept at low levels (0.01 mM) in the assay mixture. Hexokinase PI activity is not affected either by Mg2+-free ATP nor by free Mg2+ in the assay mixture. Thus, hexokinase PI and PII activities appear...
Hexokinase-Mitochondria Interaction Mediated by Akt Is Required to Inhibit Apoptosis in the Presence or Absence of Bax and Bak, ArtículoThe serine/threonine kinase Akt inhibits mitochondrial cytochrome c release and apoptosis induced by a variety of ...
1. 1. A simple procedure for the isolation of rat liver parenchymal cells, modified from the collagenase-hyalutonidase method of Berry and Friend, is descr... B Quistorff,S Bondesen,N Grunnet - 《Biochimica Et Biophysica Acta》 被引量: 63发表: 1973年 Highly Sensitive Simultaneous Bioluminesc...
Silencing HK2 in human HCC cells inhibits tumorigenesis and increases cell death, which cannot be restored by GCK or mitochondrial binding deficient HK2. Upon HK2 silencing, glucose flux to pyruvate and lactate is inhibited, but TCA fluxes are maintained. Serine uptake and glycine secretion are ...
The Ca2+ uptake supported by glucose 6-phosphate and ADP is inhibited by glucose and D-xylose. Half-maximal inhibition is observed in the presence of 0.4 mM glucose and 100 mM D-xylose. The transport ratio (Ca2+ transported:substrate utilized) is the same for glucose 6-phosphate and ATP....
opening of the MPTP is inhibited. This causes inhibition of conjugation of VDACs to Bax and Bak, and the cytochrome c is then retained inside the mitochondria. Thus, HK II helps guarantee a highly malignant tumor's proliferation, and its bypass from cell death, under conditions that would ot...
While we confirmed that IL-1β secretion triggered by ATP or nigericin in lipopolysaccharide (LPS)-primed macrophages is strongly inhibited by extracellular potassium, we found that PGN-induced IL-1β secretion is not affected by extracellular potassium (Figure 1C). We also observed no effect of...
It is overexpressed in most of the human cancers and has been a promising target for cancer therapy. Here, we report a novel selective HK2 inhibitor Benitrobenrazide (BNBZ), with nanomolar inhibitory potency. In vitro, BNBZ directly binds to HK2, induces apoptosis, and inhibits proliferation...
HK4 is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia ...