hexahistidine−metal assembliessubunit vaccinesFullyeffective vaccines must induce both potent humoral and cellularimmunities. Nanoparticles coencapsulating antigens and adjuvants haveshown promising advantages as subunit vaccines in many aspects. However,the low loading efficiency and complicated synthesis ...
Human Hexa-Histidine-Tagged Single-Chain Variable Fragments forBioimaging of Bacterial InfectionsThae Thae Min and Montarop Yamabhai*Cite This: ACS Omega 2021, 6, 762−774 Read OnlineACCESSMetrics & More Article Recommendations * sı Supporting InformationABSTRACT: The single-chain variable fra...
HexaHistidine (HRP) CAS号: 英文名: HexaHistidine (HRP) 英文别名: HexaHistidine (HRP) 中文名: 中文别名: CBNumber: CB35284369 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商1 HexaHistidine (HRP)化学性质 安全信息 HexaHistidine (HRP)性质、用途与生产工艺 ...
STABILITY of the Monoclonal Antibody to Hexa Histidine (H6) The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The...
Escherichia coli ribonuclease Ⅲ : affinity purification of hexahistidine- tagged enzyme and assays for substrate binding and cleavage[ J]. Methods Enzymol, 2001 (342) :143 -58.Amarasinghe, A. K., Calin-Jageman, I., Harmouch, A., Sun, W. & Nicholson, A. W. (2001). Escher...
Selective Derivatization of Hexahistidine-Tagged Recombinant ProteinsAffinity tagBioconjugationCoordinationHistidineMetal cationsProteinsCovalent modification of proteins is extensively used in research and industry for biosensing, medical diagnostics, targeted drug delivery, and many other practical applications. The...
This chapter describes a SELEX procedure for the identification of RNA aptamers against hexa-histidine-tagged proteins. For the efficient enrichment of higher affinity aptamers, the selection stringency should be gradually increased. Undesired RNA species that bind to affinity resins can be eliminated ...
A C-terminal hexahistidine-tagged TxA2 receptor has been constructed and expressed at high levels. Its ligand binding properties (in COS-7 cells) (Table II) and signaling properties (in COS cells and CHO cells) (Fig. 1) are not altered compared with wt-TxA2 receptors. It can be rapidly ...
The ubiquitous hexahistidine purification tag has been used to conjugate proteins to the shell of CdSe:ZnS quantum dots (QDs) due to its affinity for surface-exposed Zn2+ ions but little attention has been paid to the potential of His-tagged proteins for mineralizing luminescent ZnS nanocrystals...
•The presence of a hexahistidine tag resulted in nucleic acids binding more tightly to the Pr55Gagand altered the binding behavior compared to untagged Pr55Gag.•Removal of the hexahistidine tag resulted in binding curves similar to untagged Pr55Gag.•A cleavable His tag should be ...