目前我们临床实践中的经过FDA 批准的 HER2 IHC 商品化检测试剂盒均是为了识别HER2阳性乳腺癌而开发的(如:PATHWAY HER2(克隆 4B5;Ventana Medical Systems Inc., AZ, USA ),HercepTest (Dako Denmark A/S, Denmark) ,Oracle HER2 (clone CB11; L...
在耶鲁CLIA实验室,使用Leica Bond III Refine polymer DAB Detection和HER2/ErbB2 clone EP3 Epitomics 1:100,上以低pH提取40分钟(图2A)。Rimm Lab则使用1.28 μg/mL的HER2/ErbB2 29D8(克隆29D8,#2165,Cell Signaling Technology)和压力煮沸容器(PT ...
在耶鲁CLIA实验室,使用Leica Bond III Refine polymer DAB Detection和HER2/ErbB2 clone EP3 Epitomics 1:100,上以低pH提取40分钟(图2A)。Rimm Lab则使用1.28 μg/mL的HER2/ErbB2 29D8(克隆29D8,#2165,Cell Signaling Technology)和压力煮沸容器(PT Module, Lab Vision, Thermo Scientific, Waltham, MA)(图2B...
According to Roche, the HER2 (4B5) clone consistently achieves high proficiency assessment scores against other clones.
VENTANAHER2(4B5)isarabbitmonoclonalantibody (clone4B5)directedagainsttheinternaldomaino the c-erbB-2oncoprotein(HER2).HER2oncoproteinwascloned andcharacterizedbyAkiyamaetalin1986.Itisanapproximately 185kDtransmembraneglycoproteinwhichisstructurallysimilar toepidermalgrowth actorreceptor(EGFR).Theproteinis ...
The 50 resulting blocks were then batch stained with PATHWAY HER2/neu clone 4B5 rabbit monoclonal antibody using the Ventana Ultraview DAB detection kit in a Ventana BenchMark XT processor (Ventana, Tucson, AZ). The stained slides were reviewed and scored. We found no significant difference in...
The HER2 expression in these cases was then assessed using anti-HER2 clone PATHWAY HER2 4B5 and a HER2 reference antibody, clone SP3 (Spring Bioscience). RESULTS: 117 out of 241 (48%) breast tissue cases evaluated were HER4 positive for membrane staining by IHC using E200. The scoring ...
IHC staining HER2 IHC staining was carried out on the automatic Ven- tana Bench Mark Ultra system (Roche Diagnostics, Basel, Switzerland) using an automated staining protocol vali- dated for the anti-HER2/neu monoclonal antibody (Clone: 4B5, pre-dilution, Roche Diagnostics, Basel, Switzerland)....
[9]Layfield LJ, Frazier S, Esebua M, et al. Interobserver reproducibility for HER2/neu immunohistochemistry: A comparison of reproducibility for the HercepTest™ and the 4B5 antibody clone. Pathol Res Pract. 2016 Mar;212...
(4B5) rabbit monoclonal primary antibody (VENTANA pathway HER2, clone: 4B5, Roche Diagnostics) according to the manufacturer’s instructions, and, when necessary, the enhanced HER2 protocol was employed to detect low level of HER2 expression. For the enhanced HER2 protocol, OptiView DAB IHC ...