HepG2 cell doubling time is 48 hours. To passage cells, rinse cell monolayer with 1x PBS twice and add pre-warmed (37°C) 0.05% Trypsin-EDTA solution to cover the bottom of the flask; incubate for 5 – 7 minutes As cells detach, neutralize the Trypsin by adding 4x volume of ...
The activity of phosphomannomutase also decreased with prolongation of cellular doubling time. Furthermore, acetaldehyde treatment at 400 microM accelerated the inhibitory effect of cell growth compared with nontreated cells, and this condition facilitated CDT secretion from HepG2 cells. Determination ...
Fig. 2A shows that the total number of cells harvested after 7 days of culture did not differ between each experimental growth condition, suggesting that the proliferation rate of the HepG2 cell line remained unaffected by DNP treatment. This is confirmed by the fact that the doubling time of...
Human Intestinal Epithelial Cell-6细胞类似产品::QGY7701细胞、CNE-2细胞、PK (15)细胞 D341 Med细胞类似产品::A172细胞、ZR-75-30细胞、Doubling time: ~50 hours (ATCC).细胞 H-1734细胞类似产品::JM-1细胞、3T3NIH细胞、HBL-100细胞 MDA415细胞类似产品::H2135细胞、A 375细胞、HPDLF细胞 ...
2004. SNOM and AFM microscopy techniques to study the effect of non-ionizing radiation on the morphological and biochemical properties of human keratinocytes cell line (HaCaT). J Microsc 213: 20 – 28. Rosen AD. 1993. Membrane response to static magnetic fields: Effect of exposure duration. ...
VCP2 inhibited HepG2 cell proliferation in vitro, the growth of HepG2 cells treatment with VCP2 was slower compared with HepG2 cells, and the doubling time was extension with dose-dependent (Supplemental Fig. S1). The cell cycle distribution in normal and VCP2-treated HepG2 cells was ...
The equation of regression line is y=1.602*X + 23660, with R square of 0.953 and Pearson r value of 0.976. (D) Cell doubling times for each time points (x-axis) during 3D spheroid culture are shown as a bar graph. The units on the y-axis are hours. 3.2. Individual cell ...
edotinib.The detenninedsulfI rhodamine timeandcell dis— by B SRB ;cellgrowthcurve,doubling cyclephase tributionwere cancer resistance andits levelwere measured:breast protein BCRP mRNApmtein examined After bvRT—PCR,WestemblottingandⅡowcvtometry.RESULTSinducedfor 12 resistantcellline wasest出ished...
Since INS-1E cells require glucose and autocrine insulin is essential for their optimum growth [75], [84], the decreased glucose led to three-fold longer doubling time and impaired secretion of insulin upon glucose stimulus [75], [84]. Cells also loose their conical shape and are mostly ...
6D and E). At 50 μM, pepstatin A caused a significant increase in cell doubling time in the 4TO-NOS cell line (66.2 vs. 47.5, p = 0.021) (Fig. 6F), which was paralleled by a higher caspase-3 activity (1.73 fold increase, p = 0.047) (Fig. 6G). The addition of anti-Fas ...