Assays were conducted as follows. 100 μl sample were placed in a 75×12 mm polystyrene tube (Sarstedt, Newton, N.C.) and 100 μl of the solid-phase antibody added at 30 μg/assay. This was incubated at 37° C. for 18 minutes. 100 μl of the detection reagent was added in an ...
Wash the slide 3× in PBS for 5 min each. Add 100 μl Labelled Polymer, HRP (EnVision Kit) and incubate the slide at 37°C for 45 min. 6. Wash the slide 3× in PBS for 5 min each. Immerse the slide in freshly prepared DAB solution for 5–10 min. Observe the color ...
1C), indicating that antiviral treatment and suppression of viral load improved the immune exhaustion status among coinfected patients. Fig. 1 Expression of activation and exhaustion markers in patients with different rates of HBsAg decline. (A) Bar chart displays the median frequency of CD4+ and...
描述是一种有效的 hepatitis C virus (HCV) NS3 protease 抑制剂。 MDLMFCD27987900 别名阿舒瑞韦BMS-650032 英文名称Asunaprevir CAS630420-16-5 分子式C35H46ClN5O9S 分子量748.29 储存条件-20℃ 纯度≥98% 外观(性状)White to off-white Solid 单位瓶 ...
Wells were rinsed with another 200 μl of PBS and the cell suspensions were pooled (~ 500 μl/tube, final). The cells were pelleted at 4 °C, and re-suspended in 300 μl of cold PBS. The samples (in duplicate) in cold condition were immediately subjected to FACS scoring of GFP-...
(c) determining whether the exosome preparation comprises at least one hepatitis virus biomarker, wherein a determination of the presence of the at least one hepatitis virus biomarker in step (c) is indicative of hepatitis virus infection or hepatitis disease condition in the subject and wherein ...
After soni- cation, the samples were centrifuged at 13000 rpm at 4 °C for 10 min, and the supernatant was filtered using 3-kDa microcentrifuge filters, and 450 μL filtrate per sample was transferred into a clean microfuge tube containing 50 μL ACDSS (4.136 mM) as an ...
Subsequently, the cell suspension was transferred to a microcentrifuge tube and the cell samples were clarified by centrifugation at 12,000×g for 2 min at 4 °C. Next, 20 all of each supernatant was assayed for luciferase and Renilla reniformis luciferase activity as previously described. ...
[0024] 2) Heating a tube with the mixed serum in a PCR device at a temperature of 92° C. for two minutes and then promptly putting the tube in ice [0025] 3) Centrifuging the tube for 5-10 seconds [0026] 4) Sequentially performing reverse transcription and primary PCR reactions in...
One ml of serum was dispensed into a microcentrifugation tube (Eppendorf), and to this was added 40 mg of polyethylene glycol (PEG) 4,000 (Wako Pure Chemicals, Japan). After the tube was overturned repeatedly to well mix the content of the tube, it was left at 4° C. for 3 hr. ...