For immunohistochemical staining of PPARα, sections were incubated with the primary goat polyclonal antibody directed against PPARα at room temperature.RESULTS: Real-time PCR indicated that the PPARα level and expression level of CPT1A gene in hepatitis C patients lowered significantly as compared ...
Definition (NCI)The determination of the surface antibody reaction of a sample to the Hepatitis B virus. ConceptsLaboratory Procedure(T059) SnomedCT143473009,166105007,65911000 CPT86706 EnglishHepatitis B surface antibody measurement,Hepatitis B Surface Antibody Measurement,Hepatitis B surface antibody (HBsA...
After electrophoresis, the separated proteins were transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA), blocked with 10% milk for 1 h, and incubated with the primary antibody overnight at 4 °C. The secondary antibody used was a 1:2000 dilution of HRP-linked anti-IgG. ...
The liver biochemical parameters were normal, and the serological indicators suggested negative results for hepatitis viruses A, B, C, and E, as well as for Epstein–Barr virus and cytomegalovirus. The antibody test for autoimmune hepatitis was weakly positive for anti-smooth muscle antibodies. Two...
[3H]preS1, each over 10 min at 37 °C. Cells without tetracycline treatment served as control (set to 0%). Experiments were performed with solvent alone (set to 100%) and with increasing concentrations of the indicated inhibitors in the range of 0.1 to 1,000 µM at pH 7.4 and pH ...
2 DOSAGE AND ADMINISTRATION 2.1 Testing Prior to the Initiation of Therapy Test all patients for evidence of current or prior HBV infection by measuring hepatitis B surface antigen (HBsAg) and hepatitis B core antibody (anti-HBc) before initiating HCV treatment with SOVALDI [see Warnings and ...
For immunohistochemical staining of PPARα, sections were incubated with the primary goat polyclonal antibody directed against PPARα at room temperature. RESULTS: Real-time PCR indicated that the PPARα level and expression level of CPT1A gene in hepatitis C patients lowered significantly as compared...
HBsAg persisted with the high titer of anti-HBc antibody in pWHBV5-injected mice. 4. Discussion In this study, we created a series of chimeric WHV-HBV genomes based on a commonly used vector and explored hydrodynamic injection of the chimeric plasmids in BALB/c mice to study genomic factors...
Clearance of persistent hepatitis C virus infection in humanized mice using a claudin-1-targeting monoclonal antibody. Nat. Biotechnol. 2015, 33, 549–554. [CrossRef] [PubMed] 25. Sainz, B., Jr.; Barretto, N.; Martin, D.N.; Hiraga, N.; Imamura, M.; Hussain, S.; Marsh, K.A.;...
The HBcAg of the primary human hepatocytes infected with HBV was detected with an anti-HBcAg antibody by immunofluorescence. Moreover, secreted HBV was detected by measuring cell-secreted HBV DNA from the supernatants collected from the HBV-infected hepatocytes. The ability to detect HBV replication ...