Lastly, we developed quantitative methylation specific PCR (qMSP) assays for each of the 3 CpG islands to assess methylation in a larger sample size. We found that only the methylation of CG3 was significantly higher in HCC as compared to hepatitis and cirrhosis tissues. To our knowledge, ...
For this reason, quantitative real-time RT-PCR (qRT-PCR) assays are recommended as a first-line test for hepatitis E in this group of patients [127]. Both blood and stool samples can be tested by qRT-PCR with the latter consistently having higher viral loads. In patients with chronic ...
microorganisms Article Replenishment of Hepatitis B Virus cccDNA Pool Is Restricted by Baseline Expression of Host Restriction Factors In Vitro Sergey Brezgin 1,2,*, Anastasiia Kostyusheva 1, Ekaterina Bayurova 3,4 , Ilya Gordeychuk 3,4,5 , Maria Isaguliants 3,4,6,7, Irina Goptar 8, ...