The objective of this study was to determine the optimal laboratory method (activated clotting time [ACT], activated partial thromboplastin time [aPTT], chromogenic anti-Xa, or thromboelastography [TEG]) to monitor heparin in patients receiving temporary mechanical circulatory support. Methods: ...
ZnCl2 (0–25.6 μm in HBS containing 2 mm CaCl2 and 0.05% Tween 20) was injected into flow cells containing immobilized fibrinogen for 120 s at a rate of 30 μl/min to monitor association. Buffer lacking ZnCl2 was then injected for 300 s to monitor dissociation. Between runs, flow ...
Labs must determine the appropriate therapeutic range for their own APTT system Therapeutic APTT range must be calibrated for each reagent lot/instrument by determining APTT values that correlate with therapeutic UFH levels The Heparin Response Curve ...
in comparison to Hp as a positive control. We measured both the activity of the intrinsic and extrinsic anticoagulant cascade. All of the by-products were found to have greatly reduced ability to inhibit coagulation compared to Hp. The APTT...
(ab′)2 anti-mouse IgG-FITC were all purchased from Jackson ImmunoResearch Labs, West Grove, Pa. MOPC-21 mouse monoclonal IgG1 was purchased from Sigma Chemical, St. Louis, Mo. MBC196.8 mouse monoclonal anti-human PF4 was produced by and obtained from the Hybridoma Laboratory, Blood Research...