To prove this role in a gain-of-function model, we incorporated the heparin-binding domain of fibrin into a synthetic fibrin-mimetic matrix. In vivo, the multifunctional synthetic matrix could fully mimic the effect of fibrin in a diabetic mouse model of impaired wound healing, demonstrating the...
(1996b). Thrombin cleavage enhances exposure of a heparin binding domain in the N-terminus of the fibrin beta chain. Blood 88(6):2050-61.Odrljin, T. M., Shainoff, J. R., Lawrence, S. O. and Simpson-Haidaris, P. J. (1996). Thrombin cleavage enhances exposure of a heparin ...
At 4 degrees C, DS-PG core protein was uniformly distributed over the cell surface. At... Schmidt,G. - 《Journal of Cell Biology》 被引量: 434发表: 1987年 Isolation and characterization of a heparin-binding domain of cellular fibronectin The fibronectin molecule binds to fibrin (28), ...
Interaction of Fibrin(ogen) with Heparin: Further Characterization and Localization of the Heparin-Binding Site The β chain 15−42 sequence of the fibrin(ogen) E region was implicated in heparin binding [Odrljin et al. (1996) Blood 88, 2050−2061]; whether hepar... Sergiy,Yakovlev,...
Laminin, as a key component of the basement membrane extracellular matrix (ECM), regulates tissue morphogenesis. Here, we show that multiple laminin isoforms promiscuously bind to growth factors (GFs) with high affinity, through their heparin-binding dom
The nonspecific binding of heparin to plasma proteins compromises its anticoagulant activity by reducing the amount of heparin available to bind antithrombin. In addition, interaction of heparin with fibrin promotes formation of a ternary heparin-thrombin-fibrin complex that protects fibrin-bound thrombin ...
A variety of approaches, including scaffold functionalization, material surface modi- fication, and cell-based techniques, have been developed for this purpose12. For example, a fibrin scaffold loaded with vascular endothelial growth factor (VEGF) achieved more control over growth factor release and ...
In particular, the present invention concerns the treatment of thrombolytic disorders, e.g. AMI, with a combination of a tissue plasminogen activator (t-PA) variant having improved fibrin specificity and extended plasma half-life when compared with wild-type human t-PA and a low molecular weight...
These activated platelets amplify the coagulation cascade (Hoffman and Monroe III, 2001), culminating in the formation of insoluble fibrin. Upon trauma, the exposure of the thrombogenic subendothelium and extracellular matrix triggers the initiation of the coagulation cascade through the activation of ...
Emsley et al., “Features and Development of Coot”, Acta Crystallogr D Biol Crystallogr, 2010, vol. 66, pp. 486-501. Falati et al., “Real-Time In Vivo Imaging of Platelets, Tissue Factor and Fibrin During Arterial Thrombus Formation in the Mouse”, Nat Med., 2002, vol. 8, pp....