The top surface of the filter was then scrubbed gently with clean cotton swabs, and the migrated cells on the undersurface were fixed with ice-cold methanol for 15 min and then stained with 0.15% crystal violet before washing with PBS buffer (3 min × 3). The stained cells were then ...
(rQ) based on the standard curve and using the following equation: rQ = 10((Cq−b)/m), where Cq is the target quantification cycle, and b and m are the y-intercept and slope, respectively, from the Stock I-derived standard curve for each target [26]. The efficiency-corrected delta...
Figure 5. (a,b) TEM photographs of USeNPS (100 nm). 3.2.5. SEM Imaging The size of the SeNPs produced by U. lactuca is depicted in Figure 6. The SEM images of the SeNPs illustrated that they had a smooth surface, were oval to spherical in shape, and ranged in size from 24.32...
0.942–0.983 0.000 BMI 2.671 1.967–3.628 0.000 2.251 1.676–3.024 0.000 Abbreviations: OS, overall survival; PFS, progression-free survival; HR, hazard ratio; CI, confidence interval; HBsAg, hepatitis B surface antigen; AFP, alpha-fetoprotein; PNI, prognostic nutritional index; BMI, body mass ...
expression during CD95-mediated apoptosis of lymphocytes and hepatocytes J Huo1,3, S Xu1,3, K Guo2, Q Zeng2 and K-P Lam*,1 Fas-apoptosis inhibitory molecule (FAIM) is inducibly expressed in B lymphocytes and had been shown to antagonize Fas-mediated killing of B-cell lines in vitro....
Annexin V not only differentiates between apoptotic and necrotic cells, but it can also determine whether the apoptotic cell in the early or late apoptotic stage by binding to the phosphatidylserine on the surface of apoptotic cells [42]. Flow cytometric results revealed that encapsulated propolis ...
Nerve growth factor, beta polypeptide nerve growth factor, signal transducer activity /NGFB HGNC/—Cell-cell signaling development; 6112 energy reserve metabolism; 7166 cell surface receptor linked signal transduction; 7275 development; 4896 hematopoietin/ interferon-class (D200- domain) cytokine ...
Cell surface advanced glycation end product binding to RAGE was studied using PC3 cell adhesion assay. Results: Homophilic binding of RAGE was mediated by V1- and modulated by C2-domain in bead aggregation assay. Dimerisation of RAGE on the living cell surface was inhibited by heparin. Sulphate...