Inflammatory connective tissue diseases are characterized by idiopathic genesis along with disturbances in terms of cellular and humoral immunity, systemic organ failure and a chronic course of disease. Additionally, connective tissue diseases exhibit overlapping symptomatic features that render an accurate dia...
Figure 5 Detection of activated caspases in HepG2 cells transfected with hepatitis B virus X protein. Transfection of HepG2 cells is described in Figure 2. Forty-eight hours after transfection, the enzyme activity of caspase3, caspase8 and caspase9 was analyzed by spectrophotometric test. Data ...
(A) Lung tissue sections were stained for NE (a, c, e) or CINC-1 (b, d, f) and then counter-stained with haematoxylin. NE-positive cells lining the basal side of the bronchial epithelium were rarely observed in cases treated withS-maltoheptaose (a vs c, arrows). CINC-1 immuno-po...
The accumulation of misfolded proteins, encoded by genetic variants of functional genes leads to Endoplasmic Reticulum (ER) stress, which is a critical consequence in human disorders such as familial hypercholesterolemia, cardiovascular and hepatic disea
The detection of antibodies to specific antigens has been used in the diagnosis of specific disease states. For example, the presence of antibody to hepatitis A virus indicates infection with hepatitis A virus and the likelihood of immunity to subsequent infection with that virus. The detection of...
Statistical significance was determined by Fisher’s exact test. (B) Frequency in percent (%) of ANA patterns according to ICAP criteria detected on HEp-2 WT, LEDGF KO, and LEDGF/p75 OE HEp-2 cells detected in patients and BD. * = p < 0.05, ** = p < 0.01, *** = p < 0.001;...
(Quanta, BioScience, Gaithersburg, MD, USA) in a GeneAmp 5700 Sequence Detection System (Applied Biosystems, Carlsbad, CA, USA) employing PREXCEL-Q for all set up calculations [26]. Primer and probe sequences (Table 1) have been previously used in our lab. All sequences were generated ...
However, the lack of C2-domain in recombinant protein resulted in a diminiMshedeicdineos l2i0g18o,m5, xeFrOisRaPtiEoEnR R(FEVigIEuWre 1A,B). 5 of 14 (A) (B) FigureFhi1ogm.uorBepeh1ai.ldicBeabagidngdraiengggg.aret(gAioa)tniMonaosnasosamsyaeyorifocftflhtuheoerrereseccceeenpptttoo...