These authors showed that MDX1338 antibody alone effectively inhibited cancer cell proliferation and retarded tumor growth in xenograft models by antibody-induced apoptosis. Therapeutic mAb development against CXCR4 may be complicated due to conformational heterogeneity of CXCR4 (Baribaud et al., 2001)....
Cell pellets were subsequently re-suspended, and an antibody cocktail was added containing anti-phospho-Bcl2 (Ser70), Alexar Flour 555, anti-Bcl-2, and PECy5. Flow cytometry was performed by the bench top MUSE Cell Analyzer (Millipore, USA), and the percentage of the Bcl-2 activity ...
(SA-β-X-gal) activity; expression of senescence biomarkers including p16 INK4A (p16), p21 Waf1/Cip1 (p21), HMGB1, MMP-3, TNF-α, IL-6, lamin B1, and phospho-histone H2A.X (γ-H2AX); senescence-related gene expression; reactive oxygen species (ROS) levels; and mitochondrial ...
Next, membranes were washed with 5% Tween 20 in Tris HCl buffered saline and incubated with the secondary anti-rabbit immunoglobulin G-Peroxidase antibody (Sigma-Aldrich, Milan, Italy). Protein bands were visualized using Immobilon Western Chemiluminescent Substrate (Millipore Corporation, Billerica, MA...