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Prechilled acetone (acetone: sample volume = 6:1) was added to each tube before 4 h of precipitation at –20 °C and 20 min of centrifugation at 10,000g. Subsequently, 100 mM TEAB (100 µL) was added to dissolve the precipitate. This was followed by the addition of trypsin at an...
C domains were aligned (minimized RMSF of COG); relative movement of N-domain residues is reflected by color coding of histogram bars and protein representation from low movement (<2 Å, green) to >2.5 Å (red). (B) Cα (orange) and COG (blue) RMSF comparison of the N-intra-...
Furthermore, we also found that FXT upregulated matrix metalloproteinases (MMPs) genes, increased the protein expression of MMPs, urokinase-type plasminogen activator (uPA), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), activator protein 1 (AP-1), phosphorylated mitogen...
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