https://labblog.uofmhealth.org/rounds/early-detection-early-treatment-for-hepatitis-c Google Scholar 16. E. Gupta, M. Bajpai, A. Choudhary Hepatitis C virus: Screening, diagnosis, and interpretation of laboratory assays Asian Journal of Transfusion Science, 8 (1) (2014), pp. 19-25 View ...
Standardisation of AAB testing by IIF remains a crucial issue in and between routine laboratories and may be improved by automated interpretation systems. A novel automated reading system provides fully automated reading of fluorescence images on HEp-2 cells and software algorithms for...
IFA technology is subject to errors of interpretation and can be labor-intensive when applied to a large number of unknown samples. The ANA Hep Screen ELISA assay allows for collective and simultaneous screening for the autoantibodies of major significance in one microwell, and effectively eliminates...
of autoantigens identified from the classic autoantibody clinical test substrate HEp‑2 cells Julia Y. Wang1*, Wei Zhang2, Jung‑hyun Rho3, Michael W. Roehrl1 and Michael H. Roehrl4,5* Abstract Background: Autoantibodies are a hallmark of ...
He also performed the data analysis, interpretation, and visualisation, and wrote the manuscript. AM made the plasmid backbones that was used for the main plasmids used to make the double knockout HepG2 cells. Also provided some support when cloning the main plasmids itself. JCW performed ...
In order to facilitate interpretation, untransformed data are shown.Abbreviations IGF2BP1: Insulin-like growth factor 2 mRNA binding protein 1 miR: microRNA PARP: Poly-(ADP-ribose) polymerase RF: RISC Free TLDA: TaqMan low density array UTR: Untranslated region ...
In order to facilitate interpretation, untransformed data are shown. Additional files Additional file 1: List of proteins in the cytosolic fraction identified to be differentially expressed in HepG2 cells transfected with pre-miR-196b in comparison to cells transfected with the pre-miR negative ...
Background: The Evivar SeqHepB system is an online HBV sequence analysis tool used to determine genotype, identify antiviral resistance-associated mutations, and predict resistance to lamivudine, telbivudine, entecavir, adefovir, and tenofovir. The SeqHepB system is linked to...
(For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) 2.4. Cell proliferation and viability, nuclear area, apoptosis and cell cycle Cell proliferation was analyzed using a colorimetric ELISA (Roche Applied Science, Pe...
recommended to achieve good laboratory practice: Initial HEp-2 based screening by indirect immunofluorescence, starting with a 1:80 serum dilution, and evaluation in a bright fluorescence microscope, pathological values from a titer of 1:160 upwards, internal quality checks and unified interpretation. ...