PGC-1α)抗体(批号5)、肉碱棕榈酰转移酶1A(carnitine palmitoyl transferase 1A,CPT1A)抗体(批号1)、HRP标记的山羊抗兔IgG抗体(批号30)购自美国CST公司;核因子红细胞2相关因子2
GABPB1-AS1 regulates the erastin-induced ROS and MDA levels and abnormal expression of GABPB1 in HCC tissues. (A) HepG2 cells were transfected with siGABPB1-AS1, GABPB1-AS1 overexpression vector or the respective controls and then treated with erastin (10 μM) for 24 h. DCFH-DA ...
The membranes were incubated with primary antibodies overnight at 4 °C after blocking using a 5% nonfat milk solution for 1 h. Next, secondary antibody was used to incubate the blots under room temperature for 1 h. Finally, reactive protein was evaluated using Image Lab analysis software ...
Hepatic macrosteatosis, defined as abnormal lipid accumulation in hepatocytes in the form of large lipid droplets, predisposes to primary non-function following transplantation and is one of the most common causes for a donor organ removal from the donor pool [2]. Although a fatty liver can ...
cells Article A Multi-Omics Study Revealing the Metabolic Effects of Estrogen in Liver Cancer Cells HepG2 Minqian Shen 1, Mengyang Xu 1,2, Fanyi Zhong 2, McKenzie C. Crist 1, Anjali B. Prior 1 , Kundi Yang 2, Danielle M. Allaire 1, Fouad Choueiry 3,4, Jiangjiang Zhu 3,4,* ...