Briefly, HepG2 cells were cultured in a 24-well plate at density of 1 ˆ 105 cells/well. After starvation, cells were treated with a series of various concentrations of quercitrin in the presence of 10 mM APAP for 24 h and then incubated with 5 mg/mL MTT for additional 4 h. The ...
crocea roe as a functional food additive. Keywords: large yellow croaker roe; DHA-PC; hyperlipidemia; regulatory effect; mechanism 1. Introduction Fish roe has a high percentage of phosphatidylcholine (PC) [1,2], which has been shown to be effective for the improvement of learning ability and...
effect on HepG2 and the low hepatic lesion, being superior to paclitaxel, the positive control (LO2, 24h, IC50 = 28.5 µg/mL). [37] Among them, compound 10, the lowest inhibitor of LO2 (IC50 = 198 µM), was superior to compounds 4 and 7 in its toxicity on LO2. In addition...
The total separation for positive and negative ionization modes was 20 min. at a flow rate of 0.300 mL/min., with the HPLC gradient separation running for 11 min, followed by a 9-min wash to avoid potential carryover. Targeted data acquisition was performed together with multiple authentic ...
HepG2 Cell Culture The HepG2 cells were cultured in T-12.5 flasks and passaged at 70% confluence, using a basal medium consisting of DMEM supplemented with 10% FBS and 2% P/S, and in a 10% CO2/90% air atmosphere. The cells were then transferred into 6-well plates and grown to ...